马唐是危害最为严重的禾本科恶性杂草之一，广泛分布于全国各种作物田，严重影响作物的产量和品质。莠去津是甘蔗田除草剂的当家品种，但目前已发现甘蔗田马唐对莠去津产生抗性。本项目运用量子点荧光标记技术检测莠去津在敏感和抗性马唐中吸收和传导的差异；利用气质、液质联用仪，通过与莠去津及代谢产物标准品比对、与NIST/Willey 谱库标准谱图匹配等方法鉴定代谢产物，比较莠去津在敏感和抗性马唐代谢途径的差异性；使用SDS-PAGE比较差异蛋白，使用基质辅助激光解析电离飞行时间质谱对蛋白质进行鉴定；使用RT-PCR技术，获得不同生物型马唐psbA基因的全长cDNA，通过克隆、测序、比对，确定psbA基因的突变位点。本项目的研究结果不仅可揭示马唐对莠去津的抗药性机理，而且为延长农药的使用寿命和科学控制农田草害提供理论依据，应用前景广阔。

Digitaria sanguinalis (L.)Scop. is one of the most serious noxious weeds and spreads extensively in different crop of China. Studies found Digitaria sanguinalis (L.)Scop. have developed resistance to atrazine in sugarcane. In this study, absorption and translocation of atraizne were determined using luminescent quantum dot technology in vivo. To elucidate metabolism pathyways, metabolites of atrazine were identified with HPLC,GC-MS and LC-MS. We were compared with different protein using SDS-PAGE and identified protein with MALDI-TOF-MS. DNA sequence analysis of the chloroplast psbA gene encoding the D1 protein of PS II, the target site of PS II inhibitors, identified a point mutation in the atrazine-resistant population.The results showed resistance mechanism,provided theoretical basis for prolonging life of herbicides and controlling weed in crop.