水稻生物钟基因OsLUX参与光周期开花调控的分子机制研究

生物钟是植物的中央处理器，几乎参与调控植物所有的生长发育、应激反应及物质代谢过程，其中又以调控光周期开花研究的最深入。拟南芥夜间复合体EC（LUX-ELF3-ELF4）在调控生物钟输出途径的开花过程中起重要作用。lux、elf3和elf4突变体表现为早花，说明EC复合体抑制开花。申请人通过对粳稻日本晴进行EMS诱变获得了一个极端晚花材料，通过Mut-map技术发现拟南芥生物钟基因LUX的水稻同源基因OsLUX极有可能是目的基因，随后通过转基因全长互补、过表达和CRISPR基因敲除实验验证了其功能。由此，水稻中可能存在类似于拟南芥的EC复合体（OsLUX-OsELF3-OsELF4）调控开花。本项目拟利用qRT-PCR结合遗传实验探索OsLUX在水稻光周期开花调控中的位置，研究EC复合体存在的可能性，筛选OsLUX的互作蛋白及调控的下游靶基因，最终阐明OsLUX促进水稻光周期开花的分子机制。

The biological clock is the central processing unit of plants, which is involved in almost all the processes of growth and development, stress response and material metabolism. Among them, the most in-depth study is to regulate photoperiodic flowering. The Arabidopsis evening complex EC (LUX-ELF3-ELF4) plays an important role in regulating the flowering process of the clock output pathway. The mutant of lux, elf3 and elf4 showed early flowering, indicating that EC complex inhibited flowering. The applicant obtained an extreme late flowering material by EMS mutagenesis of japonica rice Nipponbare. It was found that the rice homologous gene OsLUX of Arabidopsis biological clock gene LUX was most likely the target gene by Mut-map technology, and then its function was verified by full-length complementation and CRISPR experiment. Therefore, there may be an EC complex (OsLUX-OsELF3-OsELF4) in rice like Arabidopsis to regulate flowering. In this project, qRT-PCR combined with genetic experiment is proposed to explore the position of OsLUX in rice photoperiodic flowering regulation, study the possibility of EC complex, screen the interaction protein of OsLUX and its downstream target gene, and finally clarify the molecular mechanism of OsLUX promoting rice photoperiodic flowering.