11C-N-CH3-Dopamine心肌Micro PET/CT显像对糖尿病心脏自主神经病变的评估

心脏自主神经病变是糖尿病慢性并发症中最严重的病变，是糖尿病患者死亡率增高的独立危险因素。糖尿病自主神经病变可表现为多种心血管功能异常，心脏自主神经反射试验，心率变异性检查等方法不能早期发现自主神经功能障碍，而核素心脏自主神经显像可在无临床症状的发病初期, 从体外无创评价心脏自主神经的微小变化。本课题拟通过心脏自主神经PET显像方法从糖尿病模型建立到心肌缺血出现为止的时间段内动态观察糖尿病模型大鼠在制模和病程进展的整个过程中放射性标记的类神经递质显像剂（交感神经显像剂11C-MDA和副交感神经显像剂11C-Donepezil）在模型大鼠心脏自主神经末梢的摄取和储存情况，评估在糖尿病病程中心脏整体和局部自主神经损伤情况和变化。找出糖尿病病程与心脏神经损伤之间的关系，进一步确定糖尿病导致心脏自主神经损伤的时间窗，对糖尿病引起心脏自主神经损伤的早期诊断提供影像学依据。

Cardiac autonomic neuropathy is the most serious disease in chronic complications of diabetes, and is an independent risk factor for increased mortality in patients with diabetes. Diabetic autonomic neuropathy can manifest as a variety of cardiovascular dysfunction. The cardiac autonomic reflex test, heart rate variability test and other methods can not detect autonomic nerve dysfunction early. Nevertheless, the radionuclide cardiac autonomic nerve imaging can be used in the in vitro noninvasive evaluation of small changes in cardiac autonomic nerves at asymptomatic early stages. This project intends to use a cardiac autonomic nerve PET imaging method to dynamically observe the uptake and storage of the radiolabeled neurotransmitter imaging agents (sympathetic nerve imaging agent 11C-MDA and parasympathetic nerve imaging agent 11C-Donepezil) in the autonomic nerve endings of diabetic model rats during the entire period from modeling to the development of myocardial ischemia to assess the overall and local autonomic nerve damage and changes in the course of diabetes. The goals are to find out the relationship between the course of diabetes and cardiac nerve damage, and determine the time point of cardiac autonomic nerve damage caused by diabetes, and provide imaging proof for the early diagnosis of cardiac autonomic nerve damage caused by diabetes.