卵巢癌对铂类耐药已经成为其治疗中的难题。切除修复交叉互补基因1（ERCC1）和乳腺癌易感基因1（BRCA1）的表达水平与顺铂化疗敏感性呈负相关。本项目前期研究发现多摄入十字花科蔬菜能够预防多个恶性肿瘤的发生并改善其预后。吲哚-3-甲醇（I3C）和异硫氰酸酯中的莱菔烷胺（SFN）作为其主要生物活性成分，能够抑制肿瘤细胞生长和分化并诱导其凋亡。本研究拟先通过体外实验研究SFN和I3C能否通过调控ERCC1和/或BRCA1的表达，增强卵巢癌细胞对顺铂敏感性的作用机制；同时通过前瞻性研究，调查经手术确诊并接受顺铂为基础化疗的卵巢癌患者，研究其肿瘤诊断后十字花科蔬菜摄入及其尿液中代谢产物与顺铂化疗应答之间的关系。本项目将体外实验和流行病学研究有机地结合，研究结果不仅能够明确十字花科蔬菜增强卵巢癌顺铂化疗敏感性的作用机制，而且为制定卵巢癌患者诊断后的膳食指导意见奠定研究基础，对改善其预后具有重要意义。

Platinum-based chemotherapy resistant has become one of the tough questions in the treatment of ovarian cancer. Recent evidence demonstrated that the expression of excision repair cross-complementation group 1 (ERCC1) and breast cancer susceptibility gene 1 (BRCA1) was inversely associated with the response to cisplatin-based chemotherapy. Our previous findings have provided evidence that high cruciferous vegetable intake may reduce the risk of certain cancers (e.g., lung cancer, colorectal cancer, and gastric cancer) and improve the survival of female lung cancer. Sulforaphane (SFN) as well as indole-3-carbinol (I3C), the major hydrolysis products and bioactive compounds of cruciferous vegetables, are involved in the inhibition of cancer growth and proliferation as well as the induction of apoptosis. Based on these findings and hypothesis, firstly, we are proposed to investigate whether SFN and I3C of cruciferous vegetables may enhance the sensitivity of human ovarian cancer cell to cisplatin through regulating the expression of ERCC1 and BRCA1 by in vitro experiments. Meanwhile, we will carry out a prospective cohort study including surgery confirmed incident cases of ovarian cancer with cisplatin-based chemotherapy identified from the Shengjing Hospital of China Medical University. On the basis of this cohort, we will investigate the associations between post-diagnostic cruciferous vegetables intake and the metabolite of cruciferous vegetables in the urinary and the response to cisplatin-based chemotherapy. Taken together, the findings of this project will not only contribute to understanding the potential mechanisms of beneficial effect of cruciferous vegetables on the response to cisplatin-based chemotherapy of ovarian cancer but also provide evidence and support the development of post-diagnostic dietary suggestions for these patients, which has important clinical implications for enhancing the sensitivity of cisplatin-based chemotherapy and improving the survival of ovarian cancer patients.