岩藻糖基化人乳寡糖的多样化酶法合成及其初步抗病原微生物黏附功能研究

岩藻糖基化人乳寡糖在婴儿健康发育中发挥着至关重要的生物学功能，但目前人乳寡糖的相关生物学研究仍然受限于结构确定的糖链样品的来源问题。基于团队前期发展的酶法模块化组装策略，本项目拟采用理性设计的酶法模块化组装策略，对线型2-位及3-位岩藻糖基化系列人乳寡糖及其唾液酸化衍生物进行多样化、系统性大量合成，首次实现部分长链及复杂岩藻糖基化人乳寡糖的合成，并初步研究目标糖链在抗病原微生物黏附方面的功能。项目研究成果将有望解决岩藻糖化人乳寡糖及其唾液酸化衍生物大量获得的瓶颈问题，为其治疗性研究提供新的思路和解决途径。. 主要内容包括：（1）酶法模块的构建；（2）运用理性设计的酶法模块化组装策略进行2-位及3-位岩藻糖基化寡糖及其唾液酸化衍生物的高效、大量合成；（3）初步研究目标糖链在抗病原微生物黏附中的功能和构效关系。

Fucosylated human milk oligosaccharides (HMOs) play pivotal roles in newborns. However, current biological studies of HMOs are hampered by the challenges posed to the large-scale production of HMOs. To tackle these problems, a diversity-oriented enzymatic modular assembly strategy will be applied for the systematic large-scale synthesis of linear 2- and (or) 3-fucosylated HMOs and their sialylated derivatives. Most of the target complex HMOs structures will be synthesized for the first time. Preliminarily study of antimicrobial adhesion function of the target glycans will be conducted. The research results obtained will help to provide new solution for the therapeutic study of fucosylated HMOs.. The main aims of this project are (1) Construction of the enzyme modules for highly efficient HMOs assembly. (2) Diversity-oriented parallel synthesis of fucosylated HMOs and their sialylated derivatives using enzymatic modular assembly strategy. (3) Preliminary study of antimicrobial adhesion function of the obtained HMOs.