渗滤液中Cl/Br代DOM的分子特征及其在生物处理过程中的转化机制

渗滤液中存在相当量的Cl/Br代溶解性有机物（DOM），源于生活垃圾带入，或在填埋过程中衍生生成。Cl/Br代DOM具有生物累积与生物放大效应。但是，它们在渗滤液中的源特征不明，其在后续生物处理过程中的转化机制不清晰，环境健康风险未知，是潜在的持久性有机污染物。据此，本项目基于高分辨质谱的非靶向分析方法，建立渗滤液中Cl/Br代DOM的近全量分离手段和嵌合结构判别的分子信息学分析方法；结合化学分子指纹特征，回溯渗滤液中Cl/Br代DOM的来源，并建立相应的基础数据库；采用分子信息耦合生物信息的多元统计学方法，探索Cl/Br代DOM在生物处理过程的转化行为与赋存规律，并探寻与其转化代谢相关的微生物；选取典型Cl/Br代微量有机污染物，基于高分辨质谱碎片与同位素示踪技术，探明其分子结构在生物处理中的转化途径。为揭示渗滤液中Cl/Br代DOM的源特征及其在生物处理过程中的转化机制提供理论依据。

Cl/Br-containing dissolved organic matter (DOM) in the leachate from solid waste, brought from municipal solid waste or derived from the secondary conversion in waste landfills, can be bioaccumulated and biomagnified in the food chain. Nevertheless, owing to the difficulty in identifying the complex DOM, there are limited reports that reveal the distribution, bio-transformation and environmental health risk of Cl/Br-containing DOM in landfill leachate, while such compounds can be the candidates of persistent organic pollutants. Therefore, the aim of this project is to propose a method to acquiring the molecular information of Cl/Br-containing DOM based on non-target analysis by using high-resolution mass spectrometry, alone with determining the molecular structure. The source of Cl/Br-containing DOM will be tracked by fingerprint analysis in terms of chemodiversity. A database will be established based on these results. Additionally, the mechanism of Cl/Br-containing DOM during bio-treatment would be elucidated by the multivariate statistical analysis combining molecular information and biological information. The specific molecule-degrading microbes will also be identified. Furthermore, typical halogenated compounds will be selected to investigate the bio-transformation mechanism thereof based on fragments of high-resolution mass spectrometry and isotopic tracer method. The outcome of this project can offer a theoretical insight into the fate of Cl/Br-containing DOM during the management of solid waste leachate.