不同传粉综合征植物花蜜脂质组比较及功能生态学研究

花蜜的产生是植物演化过程中一个重要事件。花蜜在植物与传粉生物的相互作用中发挥桥梁作用，其中非糖组分的生理生态学功能是目前研究的热点。脂质在花蜜中普遍存在，是传粉昆虫不可缺少的营养物质，其组成特征与传粉昆虫的特异性密切相关。但该方面的研究目前仍极其有限。本项目将采用点面结合的策略，以我国大型植物园的栽培植物资源为依托，主要使用以色谱-质谱联用为核心的组学技术，结合蜜腺显微技术和传粉昆虫行为学的研究方法，重点比较研究不同传粉综合征植物花蜜之间以及花内蜜与花外蜜之间脂质组成的差异、花蜜主要脂类的生态学功能、以及脂质结合蛋白的组成及活性。研究将获得在植物与传粉昆虫相互作用中关键脂质种类信息，以及主要花蜜脂质的生态学功能信息。结果将为植物与传粉昆虫相互作用及其协同进化的分子机制研究提供科学依据。

The generation of nectar is an important event in the evolution of plants. Nectar plays a bridge role in the interaction between plants and pollinators. Among them, the physiological and ecological functions of non-sugar components in nectar are hot topics in current research. Lipids are ubiquitous in nectar and are essential nutrients for insect pollinators. Nectar lipidomic characteristics are closely related to the specificity of insect pollinator, but research in this area is still extremely limited. This project will rely on the cultivated plant resources of two large botanical gardens in China (Xishuangbanna Tropical Botanical Garden and Kunming Botanical Garden), mainly using omics technologies, e.g. GC/MS and LC/MS, combined with nectary microscopy and pollinator insect behavior research methods. This project will focus on the comparative study of the difference in lipidomes between the nectar of the plants with different pollination syndromes and between floral and extrafloral nectar from the same species. We will study the ecological functions of major nectar lipids, as well as the composition and activity of lipid-binding proteins in nectar. This study will obtain information on key lipids in the interaction between plants and insect pollinators, as well as information on the ecological functions of the main nectar lipids. This result will provide a scientific basis for studying the molecular mechanism of plant-pollinating insect interaction and co-evolution.