贵州濒危植物豹皮樟的遗传分化及其类黄酮含量研究

豹皮樟（Litsea coreana var. sinensis）为樟科（Lauraceae）木姜子属（Litsea）常绿灌木或小乔木，主要生长在贵州省东北部及东南部山区。其叶片富含类黄酮，是当地少数民族制茶的主要原料，深受当地百姓欢迎。然而，当前无序的滥采滥挖导致种群资源破坏严重，亟需开展现存资源的合理利与有效保护的相关研究。因此，本研究拟通过植物形态学、生物化学、分子生物学、生态学方法对贵州豹皮樟不同种群开展系统研究，主要内容包括：(1)不同种群叶片形态特征分析；(2)不同种群主要生态因子分析；(3)不同种群叶片类黄酮含量分析；(4)不同种群叶片转录组分析；(5)根据上述结果综合分析遗传分化特征，以及影响类黄酮含量的主要内在遗传因素与外在生态因素。研究结果将为贵州豹皮樟优良种质的人工定向培育与可持续利用奠定坚实理论基础。

Litsea coreana var. sinensis, belongs to Lauraceae, is an evergreen shrub or small tree mainly growing in northeast and southeast mountain areas of Guizhou province. Its leaves are rich in flavonoids, which are the main raw materials for tea making of local minorities and important medicinal resources, and are strongly welcomed by local people. However, the adverse factors such as excessive picking leaves and digging young trees led to serious destruction of population resources at present, and it is urgent to carry out research how to rational utilize and protect existing resources. Therefore, this project intends to systematically investigate of L. coreana var. sinensis from different populations in Guizhou by means of plant morphology, biochemistry, molecular biology and ecology, and the main studies are: (1) analysis of the leaves morphological characteristics of different populations; (2) analysis of main ecological factors in different population; (3) analysis of flavonoids content in leaves of different populations; (4) analysis of leaves transcriptome of different populations; (5) comprehensive analysis of the genetic differentiation characteristics, and the main internal genetic and external ecological factors affecting the flavonoid content of L. coreana var. sinensis based on the above analyses. Results of the project will lay a solid theoretical foundation for the directional cultivation and sustainable utilization of the valuable germplasm of L. coreana var. sinensis in Guizhou Province.