基于TAS2Rs/ROCK通路探究苦味中药黄芩抑制气道刚度防治哮喘的机制研究

深吸气(DI)是一种内在有效阻止气道痉挛的方法，哮喘患者却DI失能，目前尚无DI复能的治疗方法。苦味中药黄芩通过G蛋白偶联受体（GPCRs）的亚型--苦味受体（TAS2Rs）发挥扩张支气管及抗炎作用，但具体作用机制不明。研究证实DI失能与气道刚度增加有关。我们研究发现GPCRs/ROCK通路是调控哮喘气道刚度的重要通路，因此推测“黄芩阻断TAS2Rs/ROCK通路抑制气道刚度使哮喘DI复能”。本研究拟运用大鼠过敏性哮喘模型，利用肺功能仪检测气道阻力水平，构建力-位移检测设备与伺服步进电机系统测定气道力及位移变化，使用ELISA及HE染色测定炎症水平，及Western Blot法检测TAS2Rs/ROCK通路蛋白的表达水平，确证黄芩改善DI失能的药理效应，重点考察黄芩对哮喘气道主动刚度与被动刚度的抑制作用以及与TAS2Rs/ROCK通路的关联性，以期为黄芩DI复能的临床适应症提供研究证据。

Deep inhalation (DI) is an inherently effective way to prevent airway spasms, but asthmatic patients are absent. Our previous studies have found that DI disability in asthma is related to increased airway stiffness, airway inflammation increases active stiffness, mechanical strain offsets passive stiffness, and a ROCK-dependent recovery process. ROCK is highly expressed in asthma. G-protein-coupled receptors (GPCRs)/ROCK pathway is an essential pathway in asthma. Based on this, it is speculated that if the GPCRs/ROCK pathway is effectively blocked, the airway wall's stiffness can be effectively reduced, and asthma DI can be restored. Based on the theory of TCM " nourishing-purging based on visceral conditions." However, the bitter traditional Chinese medicine Scutellaria baicalensis Georgi can exert anti-inflammatory and airway expansion effects through the GPCRs subtype bitter receptor TAS2Rs; its specific mechanism has not been studied. This study intends to use a rat model of allergic asthma to confirm the pharmacological effects of Scutellaria baicalensis Georgi in improving DI disability. Based on this basis, focus on the inhibitory effect of Scutellaria baicalensis Georgi on the active and passive stiffness of asthmatic airway and its association with TAS2Rs/ROCK pathway to provide research evidence for the clinical indications of Scutellaria baicalensis DI rejuvenation.