染色质结构调控人胚胎干细胞Primed态和Naive态多能性转化的机制研究

原始态（Naive）人胚胎干细胞（hESCs）与激发态 (Primed)相比拥有更高的多能性及稳定性，因此Naive hESCs在再生医学及疾病建模等领域拥有更广阔的应用前景。探究Primed hESCs到Naive hESCs的转化调控机制不仅能够更深入的解析胚胎发育的过程，还能够为获得优质的可应用于临床医疗的Naive hESCs提供理论基础。本研究采用小分子化学诱导方法获取Naïve hESCs，利用新的染色质结构捕获技术Dip-C结合Chip-seq、RNA-seq从基因表达、组蛋白修饰、染色质3D结构全面解析染色质结构调控Primed hESCs和Naïve hESCs的多能性转化机制。我们的目标是解析染色质调控Primed hESCs和Naïve hESCs的多能性转化机制，为干细胞应用及胚胎发育的学研究提供理论基础。

Naive human embryonic stem cells (hESCs) have higher pluripotency and stability than primed hESCs. Therefore, Naive hESCs have broader application prospects in the fields of regenerative medicine and disease modeling. Exploring the regulatory mechanism of the transformation of Primed hESCs to Naive hESCs can not only analyze the embryonic development process in depth, but also provide a theoretical basis for obtaining high-quality Naive hESCs that can be used in clinical medicine. In this study, Naïve hESCs were obtained by small molecule chemical induction method, and the new chromatin structure capture technology Dip-C combined with Chip-seq and RNA-seq was used to comprehensively analyze chromatin structure regulation from gene expression, histone modification, and chromatin 3D structure. The pluripotency transformation mechanism of hESCs and Naïve hESCs. Our goal is to establish a comprehensive detection standard for hESCs in naïve state and provide a reference for the clinical application of hESCs. Analyze the pluripotency transformation mechanism of Primed hESCs and Naïve hESCs that chromatin regulates, and provide a theoretical basis for the study of stem cell applications and embryonic development.