热胁迫下香菇DnaJ调节生长素IAA合成影响菌丝耐热性的机理研究

热胁迫影响香菇（Lentinula edodes）菌丝生长，高温常导致香菇在栽培中出现菌丝凋亡或菌棒腐烂。前期研究发现热胁迫下香菇体内一种重要的环境胁迫响应因子LeDnaJ07可通过调节胞内生长素IAA含量影响菌丝耐热性，但热胁迫下LeDnaJ07如何作用于IAA合成途径进而影响菌丝耐热性的机理尚不清楚。基于香菇基因组测序信息，本项目拟通过代谢流分析、蛋白互作、蛋白稳定性等研究内容，确定香菇体内生长素IAA合成途径及关键酶，揭示LeDnaJ07作用生长素IAA合成途径的关键靶标蛋白及其作用模式，明确LeDnaJ07在热胁迫下调控关键靶标蛋白影响耐热性的机制。本项目研究有利于揭示香菇耐热性机制，为进一步开展香菇耐热品种选育奠定理论基础，具有重要的科学意义和实践价值。

Heat stress affects mycelia growth, even high temperature often leads to the mycelia apoptosis or log rot in Lentinula edodes cultivation. Previous studies by applicants had found that the thermotolerance of L. edodes could be affected by auxin IAA which was regulated by LeDnaJ07. But how LeDnaJ07 acts on IAA synthesis pathway and affects the thermotolerance of mycelium under heat stress is unclear. Based on the genomic resequencing information of L. edodes, in this project, metabolic flow, protein interaction and stability analysis would be adopted to determine IAA synthesis pathway and key enzymes in L. edodes, reveal the key target proteins and its regulation mode of LeDnaJ07 acting on IAA synthesis pathway, and clarify the mechanism of LeDnaJ07 regulating thermotolerance by interaction with key target proteins under heat stress.This project is of great scientific significance and practical value to profoundly reveal the mechanism of thermotolerance in L. edodes under heat stress, and lay a theoretical foundation for further breeding of heat-resistant varieties of L. edodes.