PUFAs摄入及代谢对女性生殖健康与助孕治疗结局的影响及分子机制研究

生殖健康事关国计民生。目前育龄人口生育力下降，保障生殖健康已成为我国公共卫生的重大挑战。近年来膳食因素对生殖健康的影响备受关注，但膳食多不饱和脂肪酸（PUFAs）对生殖健康的影响尚不明确。尚缺乏PUFAs对育龄女性生殖健康、不孕女性生育力与辅助生殖技术（ART）治疗效果影响的人群流行病学及机制研究证据。本课题拟基于寻求ART治疗的女性人群开展跟踪随访。调查膳食PUFAs摄入，采用气相色谱法测定血清、卵泡液中PUFAs水平，综合评价PUFAs对基础性激素、卵巢保留功能、临床妊娠率、活产率等效应指标的影响，明确PUFAs摄入与代谢对女性生殖内分泌水平及ART疗效的影响。在此基础上结合靶向代谢组学技术，探明PUFAs影响女性卵巢功能的关键分子通路。项目预期在“暴露-反应”关系基础上为制定女性生殖健康膳食模式提供新线索，为通过PUFA膳食调节预防不孕不育、提高助孕成功率提供新的科学依据。

Reproductive health is crucial to national welfare and the people’s livelihood. Along with the current fecundity declining, it has become a significant challenge for public health to protect reproductive health. Recently, the influence of dietary factors on reproductive health has attracted much attention, however, the effects of dietary polyunsaturated fatty acids (PUFAs) intake remains unclear. Population-base epidemiological studies or mechanism studies are limited referring to the effects of PUFAs on female reproductive health and the efficacy of assisted reproduction technology (ART). This project is a prospective cohort study based on women under artificial assisted reproduction (ART) therapy. Baseline dietary intake of PUFAs will be measured by food frequency questionnaires, the levels of PUFAs in serum and follicle fluid will be measured by gas chromatography. Outcomes include basal hormone levels, oocyte quality, rate of good embryo, blastocyst formation rate, clinical pregnancy, pregnancy loss, live birth, etc. The associations between these outcome indicators and levels of PUFAs will be analyzed to explore the effects of PUFAs on reproductive health and ART outcomes. Furthermore, targeted lipidomic analysis method will be used to depict the metabolism of PUFAs in serum and follicle fluid and the roles of different metabolic pathways of PUFAs related to female ovarian function will be explored. This project will disclose the association between dietary PUFAs and reproductive health among Chinese women based on “exposure-response relationship” and provide evidence for reproduction protective diet, for protection against infertility through dietary PUFAs and for improvement of the outcomes of ART.