共生菌Wolbachia与Cardinium互作对烟粉虱生殖调控机制研究

烟粉虱是世界性重大农业害虫，其体内感染的共生菌可以为宿主昆虫提供营养物质、增强宿主环境适应性和提高宿主防御能力等。前期研究发现烟粉虱共生菌Wolbachia和Cardinium与其生殖相关联，但共生菌对烟粉虱的生殖调控机制尚不清楚。本项目以烟粉虱及其共生菌Wolbachia和Cardinium为研究对象，利用昆虫生态学和生物学等技术手段，构建烟粉虱不同次生共生菌感染种群，通过比较分析烟粉虱不同共生菌感染模式下有性生殖和孤雌生殖生物学参数，明确共生菌的生殖调控表型，阐明共生菌对烟粉虱生殖的影响。比较分析共生菌互作对烟粉虱生物学参数的影响，揭示共生菌互作对烟粉虱的生殖调控机制。研究结果将为解释共生菌在烟粉虱种群中的自然流行、增强宿主生态适应性、提供营养物质和提高宿主防御能力等方面的作用和机理奠定理论基础，有期为探索“以菌防虫”防控烟粉虱等害虫的新型策略制定与技术研发提供突破点。

Whitefly Bemisia tabaci is a major global spread pest and infected with several endsoymbionts. These endsoymbionts can provide essential nutrients underlimited or unbalanced diets to their hosts, manipulating host reproductive system through a variety of strategies, and also increasing the defensive ability of their host against natural enemies and pathogens. Our previous research investigated that endosymbiont Wolbachia and Cardinium are related to their hosts reproduction, but the effects of the endosymbiont Wolbachia and Cardinium on the reproductive manipulation of whitefly is still unclear. Here we focus the interactions of whitefly and its endosymbiont Wolbachia and Cardinium. Using the methods of insect ecology and biology technology, we will set up different endosymbiont infection population of whitefly. Then we will test the parthenogenesis and sexual reproduction biology parameters of different endosymbiont infection population of whitefly and determine the reproductive manipulation phenotype of Wolbachia and Cardinium, elucidating the effects of endosymbiont on the whitefly’s reproduction. Eventually, we are going to analyse the effects of Cardinium and Wolbachia on biology parameters of whitefly, revealing the reproductive manipulation mechanism of the interaction between Wolbachia and Cardinium. The results will lay a theoretical foundation for explaining the role and mechanism of the symbiosis in the natural prevalence of whitefly population, enhancing the host's ecological adaptability, providing nutrients and improving the host's defense ability, It is expected to provide a breakthrough point for the development of new strategies and technology research and development for the control of pests such as whitefly by using bacteria to prevent insects.