新疆鼠尾草通过miR-15a-5p/miR-223影响巨核细胞GPVI信号通路调控血小板聚集/抗血栓的机制研究

新疆鼠尾草作为新疆地方特色药材不仅具有抗菌消炎的作用，也具有抗血小板聚集的疗效。课题组前期多项药物研究和临床研究以及动物实验研究将新疆鼠尾草用于抗血栓/抗血小板聚集治疗均取得较好疗效。前期研究也证实了新疆鼠尾草主要含有水溶性酚酸类和脂溶性萜类化合物，且具有较强的抗氧化和抗血栓作用，并且提出具有抗血小板聚集作用的特征化合物结构依据，同时预实验也证明了新疆鼠尾草可以调控miR-15a-5p和miR-223的表达。因此课题组假设新疆鼠尾草通过miR-15a-5p和miR-223介导的GPVI信号通路调控αIIbβ3，降低血小板的聚集，同时通过动物实验和体外实验，明确新疆鼠尾草通过miR-15a-5p调控GPVI的五个相关基因，具有抗血栓的作用。本项目的完成将为中国新疆地方特色药材应用于抗血小板/抗血栓治疗提供分子生物学的依据。

As a local characteristic medicine, Salvia deserta Schang not only has antibacterial and anti-inflammatory effects, but also has an antiplatelet aggregation effect. A number of previous pharmaceutical, clinical and animal studies in our group have shown that Salvia deserta Schang has a good effect on antithrombotic/antiplatelet therapy. Previous studies have also confirmed that the water-soluble phenolic acids and lipid-soluble terpenoids mainly contained in Salvia deserta Schang have a strong antioxidation and antithrombotic effects, and proposed the structural basis for the anti-platelet aggregation effect. In addition, preliminary experiments proved that Salvia deserta Schang could regulate the expression levels of miR-15a-5p and miR-223. Therefore, we hypothesized that Salvia deserta Schang could reduce platelet aggregation by regulating αIIbβ3 via miR-15a-5p and miR-223-mediated GPVI signaling pathway. Meanwhile, animal experiments and in vitro experiments were conducted to determine the antithrombotic effects of Salvia deserta Schang and the five target genes regulated of miR-15a-5p -mediated GPVI signaling pathway. The establish of this project will provide a molecular biological basis for the application of Xinjiang local characteristic medicine in antiplatelet aggregation/antithrombotic therapy.