蝶蛹金小蜂毒液调控寄主菜粉蝶蛹脂代谢机理

寄生蜂是重要天敌资源，被广泛应用于害虫生物防治，相关研究一直是植物保护学与昆虫学研究热点之一。本项目拟围绕“蝶蛹金小蜂毒液调控寄主菜粉蝶蛹脂代谢内在机理”之科学问题，拟采用多组学结合技术、电镜技术、基因克隆与表达、蛋白质分离纯化、抗体制备、转录与表达模式分析、生物学功能评价、生物学表型观察、RNA干扰功能验证等研究方法，以“蝶蛹金小蜂—菜粉蝶蛹”为研究体系，重点开展下列研究：1）蝶蛹金小蜂毒液对寄主菜粉蝶蛹脂代谢表型影响；2）毒液调控寄主脂代谢分子机理；3）毒液中调控寄主脂代谢的活性蛋白组分鉴定；4）毒液中调控寄主脂代谢关键活性蛋白组分功能及作用模式。最终从生理学、生物化学及分子生物学水平明确蝶蛹金小蜂毒液与寄主脂代谢互作机理，鉴定获得毒液中能调控寄主脂代谢的活性组分及编码基因，解析其功能与作用模式。最终为寄生蜂保育及利用提供帮助，并为依托天敌资源建立作物害虫绿色防控体系创造条件。

Parasitoid wasps are the importantly natural enemy resources. They are commonly applied in the biological control for crop insect pests. Studying on the parasitic wasps has always been a hotspot in the research fields of plant protection and entomology. In this proposal, we will focus on the scientific hypothesis about the internal mechanisms of the regulation by Pteromalus puparum venom on the lipid metabolism of its host, Pieris rapae pupa. The methods and technologies to be used in this proposal include omics-combination analysis, observation by transmission electron microscope, gene cloning and recombinant expression, protein purification, antiserum producing, transcript and expression profile analyses, biologically functional evaluation, biological phenotype observation, and RNA interference for functional verification. The research model of P. puparum/ P. rapae system will be as the investigated object and the experiments to be performed in this proposal are listed as below. 1) The influences of P. puparum venom leading to the phenotype changes of lipid metabolism in pupal host of P. rapae; 2) the molecular mechanisms of the venom regulation on host lipid metabolism; 3) identification of the active protein components in venom, regulating the host lipid metabolism; 4) the biological functions and action modes of the actively key components identified in venom. Finally, the mechanisms of the interaction between the venom cocktail and host lipid metabolism will be illustrated at physiological, biochemical and molecular levels, respectively. The active components regulating the host lipid metabolism will be identified from the venom, their encoding genes also will be cloned, and their function and action modes will be explored. The findings from this project will further provide such assistances to the conservation and utilization of parasitoid wasps and create favorable conditions to the establishment of the green control system of the crop pests, depending on the natural enemy resources.