干细胞异常微环境导致细胞生物学特性改变为机体处于“正虚邪实，正不胜邪”的结果。IL-6R、TNFR等介导的信号途径可能调控干细胞生物学特性。益气中药黄芪可通过“精气互化”来加强对先天之精MSCs的巩固作用，维护其生物学特性稳定，从而扶正固本，祛毒攻邪。但作用的具体物质基础及机制不清。本课题拟运用IL-6、TNF-α等因子建立MSCs发生生物学特性（形态学变化、定向诱导分化能力、干性维持、染色体畸变等）改变的异常微环境，基于受体介导的信号转导通路，利用生物分子的结构信息及分子对接技术，虚拟筛选出可结合于IL-6R、TNF-αR等的黄芪有效成分。之后验证观察黄芪有效成分对异常微环境中MSCs生物学特性变化的影响，探索其维护生物学特性稳定的物质基础及分子机制。为合理筛选、快速开发增强MSCs生物学稳定性防护的新型细胞孵育剂或临床联用中药剂型提供新的思路。

Stem cell microenvironment changes result in cell biology for the body in a “virtual reality for evil”, are too numerous to evil. The signal pathway of IL-6R and TNFR might regulate stem cell biology. Tonifying qi Radix Astragali can strengthen consolidating the innate essence and maintaining the stable biological characteristics of MSCs through the “vital essence mutual transformation”, so that supporting the healthy energy and attacking pathogenic factors. However the specific material basis of Radix Astragali playing roles effectively and mechanism is not clear. In this issue we will study ways of establishing the abnormal microenvironment of MSCs through using of factors such as IL-6 and TNF-alpha etc, which could change the biological characteristics including morphological changes, directional differentiation abilities, stemness maintenance capacity and chromosome aberration etc. Based on the receptor mediated signal transducting pathways, the effective components of Radix Astragali binding to receptor such as IL-6R and TNF-alphaR can be screened out by the structural information of biological molecules and molecular docking technology.Then we will observe the effects of well-chosen components of Radix Astragali on the biological characteristics of MSCs in abnormal microenvironment in order to explore its material basis and molecular mechanism. The study will provide new thought for rational selection and rapid development new incubating agent or clinical combined Chinese medicine drugs protecting biology stability and stemness of MSCs.