类受体激酶SlFERL调节番茄果实成熟及抗性应答的机理研究

新鲜果实是膳食结构中的重要组分，其成熟进程和抗性应答直接影响果实品质和质量安全。类受体激酶通过感受环境及发育信号，启动下游信号传递，调节植物生长发育和抗病性。前期研究中，我们发现番茄类受体激酶SlFERL在果实成熟进程中表达量持续显著上升，并可能受转录因子RIN调节，但其信号传递机制仍不清楚。本项目拟在前期工作基础上，综合运用基因过表达、CRISPR-Cas9和基因沉默技术、蛋白互作分析、显微观察等方法，探明SlFERL调节番茄果实成熟和抗性应答的功能，解析SlFERL在调节果实成熟及抗性应答过程中的互作网络，揭示其基于质膜微区蛋白动态和磷酸化修饰的信号激发机制，为研究果实成熟和采后抗病应答机理提供新线索，同时为果实品质保持和保鲜技术研发提供理论依据。

Fresh fruit are important ingredients in dietary structure of mankind, their ripening and disease-resistant responses directly affect quality formation and security of fruit. Receptor-like kinases perceive environmental / developmental signals, trigger downstream signaling and regulate growth, development and disease resistance of plants. In our previous studies, we found that SlFERL was persistently up-regulated during ripening process and it may be regulated by the transcription factor RIN, however, the related signaling events are still largely unknown. On the basis of preliminary studies, the present study aims to explore the functions of SlFERL in regulating fruit ripening and disease resistance by gene overexpression, CRISPR-Cas9 and RNAi technique in combination with protein interaction analysis and microscopic observations at multi-scales. Further studies may be carried out to dissect the interacting partners of SlFERL in regulating fruit ripening and disease resistance and uncover the machinery for the microdomain- and phosphorylation-based signaling initiation. The results may provide new evidence for investigating the mechanisms for fruit ripening and disease-resistant responses, and provide theoretical basis for quality maintenance and development of fresh-keeping techniques.