环糊精包合三丁酸甘油酯的分子机制及其产物缓释行为研究

三丁酸甘油酯作为丁酸的前体物质，是很好的丁酸来源，比丁酸盐更具高效补给肠道能量和营养的功能，但三丁酸甘油酯为液态，在使用时存在难溶于水、带有苦味和生物利用率低等缺陷，限制了其在各领域尤其是食品领域中的应用。因此，着力于改善三丁酸甘油酯的使用性能显得尤为重要，而环糊精包合技术是解决这一问题的有效途径。本项目将系统研究3种常见环糊精(α-、β-和γ-环糊精)包合三丁酸甘油酯的规律，分析不同环糊精包合的协效性，解析包合物的结构特征，进而阐明包合物的形成机制；然后，基于四段消化道的体外模型，跟踪环糊精与三丁酸甘油酯结合方式的动态变化，阐明包合物中三丁酸甘油酯的迁移扩散机制；最后，基于SD大鼠的体内模型，分析环糊精/三丁酸甘油酯包合物在真实胃肠道环境中的释放机制和载体精细结构变化，揭示环糊精包合对三丁酸甘油酯生物利用的影响机制。本项目将为提升三丁酸甘油酯的应用效果和环糊精包合技术打下坚实的理论基础。

As a precursor of butyric acid, tributyrin is a good source of butyric acid and is more efficient in replenishing intestinal energy and nutrients than butyrate. However, the liquid state of tributyrin leads to many defects during its use, such as poor water solubility, bitter taste and low bioavailability, which limits its application in various fields, especially in the food field. Therefore, it is particularly important to improve the use performance of tributyrin. Cyclodextrin inclusion technology is one of the effective ways to solve this problem. Firstly, the inclusion regularity of three common cyclodextrins (α-, β- and γ-cyclodextrin) and tributyrin will be systematically studied in this project, and the synergy of the inclusion effect of different cyclodextrins will be investigated. The structural characteristics of the inclusion complex will be analyzed as well, thus to clarify the formation mechanism of the inclusion complex. Then, based on the in vitro model of the four-stage digestive tract, the dynamic changes in the combination mode of cyclodextrin and tributyrin will be tracked, and the migration and diffusion mechanism of the embedded tributyrin will be clarified. Finally, basing on the in vivo model of SD rats, the release mechanism of cyclodextrin/tributyrin inclusion complex and the fine structure changes of the carrier in the real gastrointestinal environment will be analyzed to investigate the influencing mechanism of cyclodextrin inclusion on the bioavailability of tributyrin. This project will lay a solid theoretical foundation for improving the application effect of tributyrin and cyclodextrin inclusion technology.