褪黑素调控黄瓜低温胁迫过程中特异响应的MAP激酶的鉴定及功能研究

褪黑素（Melatonin）对提高植物逆境胁迫耐受性具有重要作用，是保护植物减轻逆境胁迫伤害的重要分子。研究发现，褪黑素通过促分裂原活化蛋白（MAP）激酶信号途径影响植物天然免疫，提高植物抗逆性，但该信号途径在褪黑素提高植物抗逆性过程中的作用机制知之甚少。本项目以黄瓜为材料，分析MAP激酶信号途径对褪黑素调控黄瓜低温胁迫过程的响应，鉴定特异响应的MAP激酶；研究特异MAP激酶在褪黑素调控植物低温胁迫过程中对活性氧（ROS）积累的影响以及H2O2对特异MAP激酶调节，明确特异MAP激酶与ROS的调节关系；探究在褪黑素调控植物低温胁迫过程中，特异MAP激酶对低温响应基因表达的调控以及对光合作用的影响。项目以MAP激酶的参与和功能探究为切入点，阐释褪黑素诱导的植物抗逆信号传导机制，揭示褪黑素调节植物低温胁迫的分子机理，为褪黑素更好地应用于植物胁迫保护提供理论支持。

Melatonin plays an important role in improving the tolerance of plants to stress, and it is an important molecule to protect plants from stress damage. Studies have found that melatonin affects plant natural immunity and enhances plant stress resistance through mitogen-activated protein (MAP) kinase signaling pathway, however, little is known about the mechanism of this signal pathway in the process of melatonin's improvement of plant stress resistance. In this project, cucumber was used as the material to analyze the response of MAP kinase signaling pathway to melatonin's regulation of low temperature stress in cucumber, and to identify the specific response MAP kinase. To study the effects of specific MAP kinase on the accumulation of reactive oxygen species (ROS) and the regulation of H2O2 on specific MAP kinase in the process of regulating low temperature stress of plant by melatonin, clarify the regulatory relationship between the specific MAP kinase and ROS. To investigate the specific MAP kinase’s regulation of gene expression in response to low temperature stress and effects on photosynthesis in the process of melatonin regulating low temperature stress in plant. The project takes the participation and functional exploration of MAP kinase as an entry point, the melatonin-induced plant stress-resistant signaling mechanism is explained, and the molecular mechanism of melatonin regulating plant low temperature stress is revealed, which provides theoretical support for the better application of melatonin in plant stress protection.