卵形鲳鲹体表黏膜菌群对刺激隐核虫侵染的响应及作用

刺激隐核虫病严重危害我国南方深水网箱养殖海水鱼类，每年造成数亿的经济损失。大量研究表明，宿主黏膜菌群在寄生虫的传播、感染和疾病发展中起着重要的抑制或促进作用。皮肤和鳃是鱼类的重要黏膜界面，同时也是刺激隐核虫的主要寄生部位，然而鱼类皮肤和鳃上的黏膜菌群在刺激隐核虫病中的作用研究尚属空白。鉴于当前日益严重的刺激隐核虫感染问题，本研究以卵形鲳鲹体表（皮肤和鳃）黏膜菌群为主要研究对象，采用16S rRNA高通量测序技术分析体表黏膜菌群的群落结构与刺激隐核虫病发生、发展、易感/抗性之间的关系；并借鉴培养组学方法，对体表黏膜细菌进行高通量分离培养，然后通过共培养阻动实验和载玻片趋化实验对细菌进行快速筛选后，通过在体试验确定对刺激隐核虫病死亡率有显著提高/降低作用的细菌种类，最终阐明卵形鲳鲹体表黏膜菌群对刺激隐核虫感染的响应及其作用。本研究结果将为开发刺激隐核虫病的防治新方法提供新的思路和理论基础。

Cryptocaryoniasis seriously damaged the offshore-cage culture of marine fish in South China, causing hundreds of millions of economic losses every year. Numerous studies showed that, the host microbiota at mucosal interfaces can play an important role in the transmission, infection of parasites, preventing or contributing to the progress of parasite diseases. The skin and gill are important mucosal interfaces in fish. Meanwhile, they are also the major invasion sites of Cryptocaryon irritans. However, majority of the existing researches on cryptocaryoniasis centered around the host-parasite, ignoring mucosal microbiota at skin and gill. In view of the increasing problem of C. irritans infection in Trachinotus ovatus cage farms, this study characterizes the microbiome composition and structure of the mucosal surfaces on the skin and gill of T. ovatus by high-throughput sequencing of metagenomic 16S rRNA, so that the potential roles of the mucosal microbiota in the invasion, infection of C. irritans, and its relationship with host’s susceptibility / resistance to cryptocaryoniasis could be explored. The host mucosal bacteria will also be isolated by various culture conditions, isolates bacteria that could improve / reduce the mortality of C. irritans will be determined by in vitro and in vivo tests. Results of this study will provide theoretical basis for developing new methods to control cryptocaryoniasis.