介导两种舟蛾生殖隔离的信息素受体选择性识别机制

信息素受体（PR）作为昆虫识别性信息素的关键蛋白，是决定雄蛾能否准确识别配偶的关键因子。申请人前期研究发现，杨小舟蛾和仁扇舟蛾常混合发生，且存在高度保守的PR基因，表明二者信息素组分具有相关性，但田间诱捕实验中无交叉引诱现象。故推测两种舟蛾的PR对性信息素组分的选择性识别是维持二者种间生殖隔离的重要原因，但其选择性识别机制尚不清楚。本项目已通过转录组测序及系统发育分析完成了两种舟蛾PR基因鉴定；拟采用分子对接模拟两种舟蛾PR与已报道舟蛾类性信息素成分的结合，根据结合能力初步筛选对杨小舟蛾性信息素组分Z13,E15-18:Ald具有结合能力的PR，并反向推导仁扇舟蛾性信息素活性成分的化学结构；最后结合异源表达、RNAi、触角电生理以及行为学实验，对筛选出的PR进行生理功能研究，阐明其对性信息素的选择性识别机制。从化学生态学角度揭示介导两种舟蛾生殖隔离的分子机理，为开发绿色防控技术提供新思路。

Pheromone receptor (PR) is a key factor in mate recognizing, since they play important role in pheromone detection of male moth. The applicant found that Micromelalopha troglodyta and Clostera restitura often appear together, and share several highly conservative PRs, indicate the relevance in pheromone components, nevertheless, no overlap was found in field experiments. Suggests that the selective detection of PRs to pheromone components is a significant cause to the maintenance of reproductive isolation, however, the selective detection mechanism is unclear. In this study, PR genes have been identified by RNA-Seq and phylogenetic analysis; Molecular docking will be applied to simulate the binding of PRs to pheromone components and their isomers from Notodontidae, in order to find out the specific PR which involved in Z13,E15-18:Ald detection, and to predict the potential pheromone components of C. restitura; Then the physiological function of pheromone receptors will be uncovered in vitro and in vivo to explore the selective detection of pheromone. The results will reveal the selective detection mechanism of sex pheromone that mediate the reproductive isolation of two Notodontidae species and provide a new idea for developing green control techniques.