热休克蛋白70（Hsp70）参与ALV-J病毒颗粒组装的分子机制研究

J亚群禽白血病是由ALV-J引起的严重危害养禽业的肿瘤性疾病。虽然ALV-J的感染与防控受到了广泛关注，但ALV-J生命周期与致病机理尚不十分清楚。本团队首次发现ALV-J病毒颗粒和病毒蛋白Gag形成的病毒样颗粒中包装着宿主蛋白——热休克蛋白70（Heat shock protein 70, Hsp70），改变细胞内Hsp70的含量或功能会影响ALV-J病毒颗粒的组装，进而影响病毒的增殖。Hsp70在细胞生命周期和病毒增殖过程中具有重要作用，因此探明Hsp70参与ALV-J病毒颗粒组装的分子机制具有重要意义。在此基础上，本项目重点研究Hsp70与ALV-J病毒核心蛋白相互作用对病毒颗粒组装的影响以及Hsp70氨基酸位点生化修饰对ALV-J复制过程的影响，阐明Hsp70在ALV-J病毒颗粒组装中的作用和分子机制，为解析ALV-J复制过程以及防控禽白血病提供依据。

Subgroup J avian leukosis caused by subgroup J avian leukosis virus (ALV-J) is an infectious neoplastic disease which has been seriously plaguing poultry industry. In our previous study, a novel host protein heat shock protein 70 (Hsp70) was identified in the mature viral particles and the virus-like particles formed by Gag protein, affecting viral replication in host cells. However, the role of Hsp70 in the viral particle assembly and the underlying molecular mechanism is still unclear. Considering the critical role of Hsp70 in cellular life cycle and viral replication, it was important to determine the role of Hsp70 in viral assembly during ALV-J replication. Based on our previous findings, this project will deeply explore the interaction between Hsp70 protein and viral proteins and the effect of such interaction on viral assembly, and elucidate the effect of Hsp70 protein modification on viral replication. The findings from this study will help to provide theoretical guidance for understanding on the life cycle of ALV-J infection.