AM真菌介导的MAPK和Msn2对杨树菌根耐旱基因的分子调控

干旱是土地荒漠化加剧、水土流失严重的最重要因素之一，严重制约着生态环境建设和植被恢复，提高干旱、贫瘠等恶劣条件下的造林成活率，破解菌根真菌提高植物抗旱性的分子机制就显得尤为重要和紧迫。本项目在研究团队前期菌根真菌调控林木磷转运蛋白和水孔蛋白相关基因研究的基础上，选择干旱地区主要造林树种和优势菌根真菌，深入研究干旱胁迫下AM真菌响应干旱胁迫的MAPK信号相关基因和Msn2转录因子编码基因的功能。从转录因子层面上研究和挖掘基因及其功能，分析AM真菌MAPK途径与Msn2转录因子的关系，MAPK和Msn2与耐旱密切相关的水孔蛋白和磷转运蛋白基因的相关性，揭示AM真菌介导的林木菌根耐旱基因的分子调控作用。为菌根真菌增强林木耐旱、加快逆境森林恢复的生态调控奠定理论基础。研究结果对干旱荒漠区AM真菌提高林木造林成活率，促进森林恢复和生态修复，具有十分重要的理论价值与实践意义。

Drought is one of the major reasons for aggravated land desertification and severe soil erosion. Increment of the afforestation survival rate under severe conditions including drought and barrenness, and deciphering the molecular mechanism that mycorrhizal fungi improve plant drought tolerance appears very important and urgent. Based on the mycorrhizal fungi mediated phosphate transporter and aquaporin-related genes studies of our research team, the project will take the main afforestation species and dominant mycorrhizal fungi in arid area to further investigate the MAPK signaling related genes as well as the function of Msn2 transcription factor encoding gene of AM fungi in response to drought stress. The project will in the transcriptional factor level decipher and explore genes and their function, analyze the relationship between AM fungal MAPK pathway and transcriptional factor Msn2, assess the correlation amongst MAPK, Msn2, and drought-tolerance-related phosphate transporter and aquaporin genes, unveil the molecular regulation of AM fungi-mediated drought-tolerance genes of mycorrhiza in forest trees, and lay a theoretical foundation for the ecological regulation of mycorrhizal fungi enhancing drought tolerance and accelerating forest restoration in adversity. The results will be of great importance in theoretical value and practical significance for increasing the survival rate of afforestation, promoting forest recovery and ecological remediation through application of AM fungi in arid and desertification areas.