外泌体CD44v促进乳腺癌骨转移的作用机制及其作为标志物的应用研究

乳腺癌骨转移是导致乳腺癌患者死亡的重要原因，明确乳腺癌骨转移的分子机制，鉴定筛选相应分子标志物，有利于乳腺癌的诊断治疗。外泌体作为通讯信使在肿瘤转移形成中发挥着不可替代的作用，但在乳腺癌骨转移中的作用尚不完全清楚。在前期研究中，我们发现亲骨性乳腺癌细胞分泌的外泌体能够促进低恶性乳腺癌细胞转移，增强破骨细胞分化，其中外泌体中高表达的CD44v可能参与乳腺癌细胞骨转移。在本项目申请中，我们将深入研究外泌体CD44v在乳腺癌细胞与破骨细胞信息传递的方式，解析CD44v-ERM及CD44v-RANK信号通路在乳腺癌骨转移发生过程中的调控机制。项目研究结果将揭示乳腺癌骨转移新机制，鉴定筛选外泌体CD44v并探索其作为乳腺癌骨转移标志物的可能性。

Breast cancer bone metastasis is an important cause of death in breast cancer patients. To clarify the molecular mechanism of breast cancer bone metastasis, identify and screen corresponding molecular markers, is beneficial to the diagnosis and treatment of breast cancer. As a communication messenger, exosomes play an irreplaceable role in the formation of tumor metastasis, but the role of breast cancer bone metastasis is not fully understood. In previous studies, we found that exosomes secreted by osteophilic breast cancer cells can promote the metastasis of low-malignant breast cancer cells and enhance osteoclast differentiation. Among them, the high expression of CD44v in exosomes may be involved in breast cancer cell bone metastasis. In this project , we will deeply study the way of exosomal CD44v information transmission in breast cancer cells and osteoclasts, and analyze the regulatory mechanism of CD44v-ERM and CD44v-RANK signaling pathways in the process of breast cancer bone metastasis. The results of the project will reveal the new mechanism of breast cancer bone metastasis, identify and screen exosomal CD44v, and explore its possibility as a marker of breast cancer bone metastasis.