NGF在运动改善原发性痛经中的作用及机制研究

近年来的研究报道运动在原发性痛经（Primary dysmenorrhea, PD）的治疗中发挥积极作用，但其作用机制仍不清楚。课题组前期对6770名PD患者进行了全基因组关联分析及验证，发现神经生长因子（Nerve Growth Factor, NGF）基因多态性与PD存在强阳性关联。基于此，本研究进一步把NGF与PD的疼痛信号传导关键激酶（MAPK）联系起来，首次探索PD疼痛运动干预后中枢疼痛调控机制。通过建立PD小鼠模型，观察4周不同运动强度跑台运动对PD小鼠背根神经节内NGF及其受体表达、MAPK信号通路各分子表达及磷酸化水平的影响。此外，通过各组小鼠背根神经节细胞培养，使用NGF表达刺激剂和抑制剂以及RNA干扰（RNAi）技术，观察NGF表达的变化与MAPK通路各分子之间的关系。以期通过动物研究和细胞实验的结合，阐明NGF在运动改善PD疼痛中的作用及分子机制。

Recent studies have reported that exercise may play an active role in the treatment of primary dysmenorrhea (PD), but its mechanism is still unclear. We conducted a genome-wide association analysis and verification on 6770 PD patients, and found that NGF gene polymorphisms were positively associated with PD. We plan to link NGF with MAPK for pain signal transduction, and explores the mechanism of central pain regulation after PD pain exercise intervention for the first time. By establishing a PD mouse model, we observed the effects of treadmill exercise with different exercise intensities for 4 weeks on the expression of NGF and its receptors in the dorsal root ganglia. The influence of the expression and phosphorylation level of each molecule in the MAPK signaling pathway. In addition, through the culture of mouse dorsal root ganglion cells in each group, using NGF expression stimulators and inhibitors, and RNA interference (RNAi) technology, the relationship between the changes in NGF expression and the molecules in the MAPK pathway was observed. It is hoped that through the combination of animal research and cell experiment, supplement the role and molecular mechanism of NGF in improving PD pain by exercise.