HBV上调的hsa_circ_0007807竞争性结合miR-1249-5p调控TPX2在肝癌发生发展中的作用及机制研究

环状RNA（circRNA）在肿瘤发生发展中有重要的调控作用，但其在HBV相关肝癌中的作用尚不清楚。我们前期通过测序技术及qRT-PCR发现circ_0007807在HBV阳性肝癌细胞及组织中高表达，并可促进肝癌细胞增殖和转移能力；HBV可通过HBx诱导circ_0007807表达；circ_0007807与miR-1249-5p存在序列互补并相互负调控其表达，TPX2与miR-1249-5p也存在结合位点，且与miR-1249-5p负相关，与circ_0007807正相关。据此推测：HBV上调的circ_0007807通过竞争性结合miR-1249-5p上调TPX2的表达进而促进肝癌增殖和转移。本研究拟从细胞、病人组织及动物水平等多层次阐明circ_0007807/miR-1249-5p/TPX2轴作用于肝癌的分子机制，以期为HBV相关肝癌提供circRNA导向的诊断及治疗靶标。

CircRNA plays an important role in the occurrence and development of tumors, but its role in HBV related liver cancer is not clear. We found that circ_0007807 was highly expressed in HBV positive HCC cells and tissues by sequencing technology and qRT-PCR, and it could promote the proliferation and metastasis of HCC cells; While HBV could enhance its transcription through HBx; circ_0007807 and miR-1249-5p could not only complement each other in sequence, but also negatively regulate each other's expression, TPX2 and miR-1249-5p also have binding sites, and TPX2 was negatively correlated with miR-1249-5p and positively correlated with circ_0007807. It can be concluded that HBV-upregulated circ_0007807 competitive binding miR-1249-5p to upregulate the expression of TPX2, thus promoting the proliferation and metastasis of HCC cells. This study will further clarify the molecular mechanism of circ_0007807/miR-1249-5p/TPX2 axis acting on HCC cells, using cell, patient tissue and animal model, so as to provide a circRNA-oriented target for diagnosis and treatment of HBV related HCC.