材料表面细菌运动动力学与生物膜形成的关联机制研究

本课题拟研究材料表面的细菌运动动力学与其形成的细菌生物膜之间的定量关系，阐述两者间的关联机制，并基于此构建一种新的研究细菌生物膜调控的手段。即构建起细菌在材料表面的运动动力学行为与生物膜之间的定量模型，以实现对生物膜形成的精准定量化调控。为实现以上研究目标，本研究拟自主搭建新型单细菌追踪显微技术以获取材料表面处细菌的运动动力学数据，并观测细菌形成的成熟生物膜；对二者进行特征挖掘后，利用机器学习算法建立起定量的数学模型；建立起有效的定量模型后，本研究拟结合理论仿真对细菌在材料表面的运动动力学行为进行模拟，最终形成对生物膜调控材料制备的有效指导，并进行实验验证。本研究的实施将帮助我们进一步理解细菌生物膜的形成机制，并对抗菌、生物降解与生物合成材料的制备起提供新的理论指导和技术支持。

Bacterial motility at material surface plays important roles for the formation of biofilm. However, the underlying mechanism of how bacterial motility regulates biofilm formation remains elusive. In this proposal, I propose to study the quantitative relation between the dynamics of bacterial motility and the quantitative parameters extracted from the formed biofilms. Based on this, I propose to build statistical machine learning models between the aforementioned two. Then, I propose to simulate the active dynamic motion of bacteria on the surface of materials and find the dynamic parameters of bacterial motility from the simulation. Combining the aforementioned results, I propose to design material surficial structures and the chemical/physical environment near the surface, and finally realize accurate manipulation of biofilm formation. The proposed researches can help us better understand how biofilms are regulated by the motility of bacteria at the early stage. Also, the proposed researches can provide important guidance and support to related research/industrial field for design and fabrication of biofilm manipulation materials.