基于肠道菌群与3型天然淋巴细胞相互作用研究参苓白术散干预炎性肠病的分子机制

前期研究表明参苓白术散对断奶仔猪腹泻有显著的防治效果，对肠道菌群具有调控作用。肠道菌群多样性和丰富度变化是肠道疾病发生的主要因素，ILC3分泌的IL-17易激发炎性肠道疾病的发生，为了进一步研究肠道菌群与ILC3之间存在相互关系对炎性肠病的影响，同时探讨参苓白术散是否通过调控肠道菌群与ILC3之间的互作而发挥健脾益气、渗湿止泻的功效，本项目以IBD大鼠、ILC3基因缺失大鼠为模型，应用高通量测序技术、流式细胞技术以及q-PCR/WB等方法和手段，对肠道菌群和ILC3发育进行分析，观察肠道菌群和ILC3变化规律以及在IBD中发挥的作用，建立相互作用的调控网络，从肠道微生态和免疫学的角度阐述参苓白术散的健脾益气、渗湿止泻的分子调节机制。本研究不仅可以揭示肠道菌群影响肠道先天免疫系统的确切机制，而且还有望为未来将调节肠道菌群平衡作为临床治疗免疫相关疾病的靶点提供依据。

Previous studies have shown that Shenling Baizhu Powder has a significant preventive effect on diarrhea in weaned piglets and has a regulatory effect on gut microbiome. Changes in the diversity and richness of gut microbiome are the main factors for the occurrence of intestinal diseases. IL-17 secreted by ILC3 can trigger the occurrence of inflammatory intestinal diseases. To investigate the relationship between gut microbiome and ILC3 in inflammatory bowel disease, and exploring whether Shenling Baizhu San strengthening spleen and replenishing qi via regulating the interaction between gut microbiome and ILC3. In this study, using gene ILC3 knocked out and IBD rats as model, applied high-throughput sequencing technology, flow cytometry and q-PCR / WB methods to analyze the development of gut microbiome and ILC3. Baded on the changes of gut microbiome to establish an interactive regulatory network and elaborate the molecular mechanism of Shenling Baizhu San for strengthening spleen and replenishing qi from the perspective of intestinal microecology and immunology. This study can not only reveal the exact mechanism of the gut microbiome affecting the intestinal innate immune system, but also to provide a basis for future adjustment of the gut microbiome balance as a target for clinical treatment of immune-related diseases.