AaMYB16-AaHD1-AaMYB5复合体通过调控青蒿腺毛起始发育影响青蒿素合成的分子机理研究

植物分泌型腺毛因其特有的合成和储存次生代谢产物的能力被称为“细胞工厂”。青蒿素产生并储存于青蒿的分泌型腺毛中，提高腺毛密度可提高青蒿素含量，有助于解决全球青蒿素供应不足的问题。为此，需对青蒿腺毛的起始发育机制这一相对薄弱的领域进行研究。AaHD1是已知的青蒿腺毛起始发育的重要促进因子，但是其介导的信号转导途径未知。我们在前期工作中分离了AaHD1互作蛋白AaMYB5和AaMYB16以及AaHD1调控的下游基因AaWRKY75b，并初步证实它们都参与腺毛起始发育调控。基于此，本研究拟进一步明确这些基因调控腺毛起始发育的功能，研究AaMYB16、AaHD1、AaMYB5形成调控复合体的方式，以及它们在分子和遗传水平对AaWRKY75b进行调控的方式，从而揭示AaMYB16-AaHD1-AaMYB5复合体调控青蒿腺毛起始发育的分子机理，为探讨提高青蒿素含量的方法提供理论指导。

Glandular trichomes are known as "cell factories" for their unique ability to synthesize and store secondary metabolites in plants. Artemisinin is produced and stored in the glandular trichomes in Artemisia annua L,. Increasing the density of glandular trichomes can increase the content of artemisinin, which helps to solve the problem of insufficient supply of artemisinin in the world. Therefore, it is necessary to study the mechanism of glandular trichomes initiation and development in A. annua, where there is still a gap in our understanding. AaHD1 is a known key factor that promotes the glandular trichomes initiation in A. annua, but the signal transduction pathway mediated by AaHD1 remains elusive. In our preliminary study, we isolated the AaHD1 interaction proteins AaMYB5 and AaMYB16, as well as the downstream gene AaWRKY75b regulated by AaHD1. And they were preliminarily proved to function in the regulation of glandular trichomes initiation. Based on these analyses, this study plans to further confirm the function of these genes in glandular trichomes initiation, to explore the mode of AaMYB16, AaHD1 and AaMYB5 forming functional complex, and the mode of their regulation on AaWRKY75b at molecular and genetic level. So that reveal the molecular mechanism of the AaMYB16-AaHD1-AaMYB5 complex regulating the initiation of glandular trichomes, and provide theoretical instruction for improving the content of artemisinin.