根际促生菌Tsukamurella tyrosinosolvens P9提高花生抗旱性的分子机理研究

植物生长过程常遭遇逆境胁迫，花生种植受干旱影响大。根际促生菌可提高植物抗旱性，但迄今未有稀有放线菌-束村氏菌属(Tsukamurella sp.)的抗逆促生报道。项目前期已从茶树根际分离到Tsukamurella tyrosinosolvens P9，菌株接种可显著提高花生幼苗的抗旱性，但调控机制未知。本项目拟构建“菌株-花生”共培互作体系，分析PEG胁迫时P9菌株发挥抗逆促生功能的关键基因、过表达及接种试验以鉴定基因功能，转录组分析菌株接种的花生对干旱胁迫的响应机制；动态追踪菌株对水亏缺及复水处理花生抗旱性、生长发育及根际微生态的影响；结合微生物学、植物生理学及分子生物学等多学科技术、从生理生态功能、基因表达、信号传导及代谢途径综合解析P9菌株提高花生抗旱性的机理。项目是对已有研究的进一步深入和延续，不仅可拓展束村氏菌属的生态学功能，还可补充和丰富PGPR对植物抗逆性的多样化调控机制。

The plant suffers various adverse environmental stresses in its life cycle. Drought has much effect on the groundnut cultivation. Plant growth-promoting rhizobacteria (PGPR) could improve drought resistance of plant. However, Tsukamurella sp. has not been reported characteristics of stress resistance and growth promoting on plants up to now. In our previous study, Tsukamurella tyrosinosolvens P9 strain, isolated from tea rhizosphere, was found to enhance obviously drought resistance of peanut seedling, but the mechanism has been unknown. In this study,“P9 strain-Peanut seedling”co-culture system was constructed, the key stress-resistant genes of P9 strain should be screened under PEG treated condition, gene function be identified by overexpression and inoculation on peanut. And the drought response mechanism of peanut, treated with P9 strain, were analyzed using RNA-Seq. Influence on drought-resistance and growth of peanut, and rhizosphere microecology were studied dynamically after water deficit and rehydration. In total, combining with microbiological, plant physiological and molecular biological means, drought-resistant mechanism of peanut inoculated with Tsukamurella tyrosinosolvens P9 should be illuminated from the aspects of physiological and ecological function, gene expression, signal transduction and metabolic pathways. This study should deepen and continue our previous research further. And the result should not only expand ecological function of Tsukamurella sp., but also complement and enrich diversified regulatory mechanism of PGPR on improving stress-resistance of plant.