苹果MdWOX11调控MdLAX3介导IAA极性运输促进不定根发生的分子机制

不定根诱导是苹果无性系矮化砧木繁育的关键环节。前期工作表明，不定根发生正调控因子MdWOX11可能通过调控IAA输入基因MdLAX3介导IAA极性运输促进不定根发生，但相关分子机制尚待解析。基于此，本项目拟以MdWOX11转基因苹果为试材，检测茎基部IAA含量及分布变化，原位杂交分析MdLAX3在茎基部的分布，明确MdWOX11通过调控IAA极性运输促进不定根发生；拟通过多个试验解析MdWOX11与MdLAX3的转录调控模式，揭示MdWOX11转录调控MdLAX3介导IAA极性运输参与不定根发生的调控机制；利用苹果转基因分析MdLAX3介导IAA极性运输调控不定根发生的功能。项目旨在阐明MdWOX11调控MdLAX3介导IAA极性运输促进不定根发生的分子机制，为提高苹果生根能力促进无性系矮化砧木繁育提供理论指导。

Adventitious rooting is the key to propagating apple clonal dwarf rootstocks. Previous work has found that positive regulator of AR formation MdWOX11 may be through regulating IAA input-related gene MdLAX3 mediated IAA polar transport to promote AR formation in apple, but related molecular mechanisms remains to be resolved. Based on this, MdWOX11 transgenic apple materials was used as test materials to detect the content and distribution changes of IAA in the stem during AR formation, and in situ hybridization was used to analyze the distribution pattern of MdLAX3 in the stem, so as to determine whether MdWOX11 promotes AR formation by regulating the polar transport of IAA. In further, multiple experiments confirm that the transcriptional regulation mode of MdWOX11 and MdLAX3, explore regulation relationships of MdWOX11 and target gene of MdLAX3 to participate in AR formation in apple, and MdLAX3 mediated IAA polar transport to regulate AR formation through the apple transgenic technology that was analyzed. The purpose of the project is to clarify the functional and molecular mechanism of MdWOX11 regulating MdLAX3 mediated IAA polar transport to promote adventitious root formation in apple, and provides theoretical guidance for improving the rooting ability of apple and promoting the breeding of clone dwarfing rootstocks.