基于源库关系的核桃光合产物同化与分配格局研究

源库关系通过调节光合产物的同化与分配影响果树产量和果实品质。但是研究人员对果树光合产物同化与分配同源库比的关系看法不一（正相关、或复相关、或无关）。项目组前期研究发现，当源库比在不同范围内变动时，核桃光合产物同化与源库比的关系不同。基于此，提出假设：光合产物的同化与分配同源库比的关系取决于源库比的范围。为验证上述假设，本项目拟以新疆早实核桃主栽品种‘新新2号’为供试材料，通过摘叶或/和去果设置不同的源库比处理，以基于同位素示踪技术获得的核桃光合产物同化与分配特征为依据，运用光合特性分析、糖代谢分析、解剖结构观察 、基因表达分析、相关分析等植物生理学、生物化学、分子生物学和数理统计学的技术手段，通过对核桃不同源库比环割结果枝上叶片、果柄和果实的解剖结构、碳水化合物积累与代谢等方面的研究对假设进行验证，以期为新疆南疆盆地核桃生产园精准化栽培管理提供科学依据，丰富果树源库关系理论体系

Source sink relationship affects fruit yield and quality by regulating the assimilation and distribution of photosynthate. However, researchers have different views on the relationship between assimilation and distribution of photosynthate and the source-to-sink ratio (positive correlation, or negative correlation, or irrelevant).The preliminary study of the project team found that when the source-to-sink ratio changed in different ranges, the relationship between assimilation and distribution of photosynthate and the source-to-sink ratio was different. On the basis of the above research, a hypothesis is put forward: the relationship between assimilation and distribution of photosynthate and source-to-sink ratio depends on the range of source-to-sink ratio. For this hypothesis, this project takes Xinjiang Walnut Cultivars ‘J. regia Xinxin2’ as the research object, and applies different source-to-sink ratio by picking leaves and/or removing fruits, and takes the characteristics of the assimilation and distribution of walnut photosynthate as a basis, which was obtained through isotope tracer technology. By means of photosynthetic characteristics analysis, sugar metabolism analysis, anatomical structure observation, gene expression analysis, correlation analysis and other plant physiological, biochemical, molecular biology and mathematical statistics techniques, and the anatomical anatomy, carbohydrate accumulation and metabolism of the leaf, carpopodium, and fruit on the girdled shoots with different source-to-sink ratio are studied to verify the hypothesis, provide scientific basis for precise cultivation and management of walnut production park in South Xinjiang Basin, and enrich the theoretical system of source sink relationship of fruit trees.