3D打印功能化复合水凝胶支架用于乳腺癌治疗及脂肪再生

乳腺恶性肿瘤对全球不同年龄段的女性都会造成威胁，切除手术后造成乳腺缺损，同时可能有肿瘤细胞残余。放/化疗作为辅助疗法，会给人体带来一定毒副作用。因此，亟需构建兼具修复受损乳腺脂肪组织，同时有效抑制乳腺癌转移复发的双功能生物活性材料。本项目拟采用3D打印技术构建GelMA-树枝状介孔二氧化硅(DMSNs)复合水凝胶支架材料，其中可降解的DMSNs包裹了靶向小分子抗癌药物，通过改变复合水凝胶组成及结构实现靶向药物及无机功能离子的可控释放。阐明复合水凝胶支架在体外、体内抑制肿瘤生长、转移的效果及作用机制。并重点探究复合水凝胶支架通过可控释放的功能无机Si离子在体内促进脂肪再生、血管生成的效果，阐明复合水凝胶支架的组成及结构与肿瘤治疗、脂肪再生、血管生成之间的构效关系。最终，实现构建一种新颖的功能化复合水凝胶支架，兼具乳腺癌治疗与脂肪再生的的功效，其具有用于临床上治疗乳腺癌的潜在前景。

Breast malignancies pose a threat to women of all ages around the world. Surgery may lead to breast defects, followed by possible residual tumor cells. As adjuvant therapy treatments, radiotherapy/chemotherapy will bring certain toxic and side effects to human body. Therefore, it is urgent to construct bifunctional bioactive biomaterials that can repair the damaged breast adipose tissue after surgical resection and effectively inhibit breast cancer metastasis and recurrence. This project intends to construct a 3D printing composite hydrogel scaffold of GelMA-dendritic mesoporous silica nanoparticles (DMSNs), in which targeted small molecule anticancer drugs were integrated. Controlled release of targeted drugs and inorganic functional ions can be realized by changing the composition and structure of the composite hydrogel scaffolds. The effect and mechanism of composite hydrogel scaffolds in inhibiting tumor growth and metastasis in vitro and in vivo will be elucidated. Furthermore, the effects of controllable release of Si ions of the composite hydrogel scaffolds on promoting adipose regeneration and angiogenesis in vivo will be explored. In addition, the relationship between the composition and structure of the composite hydrogel scaffolds and tumor therapy, adipose regeneration and angiogenesis will be clarified. Finally, a novel functional composite hydrogel scaffold will be constructed, which has the efficacy of both breast cancer treatment and adipose regeneration, having a potential prospect for clinical treatment of breast cancer.