生物分子马达ATP合酶的定向组装及性能调控研究

ATP是细胞进行各种生命活动的能源物质，主要由跨膜质子梯度势驱动生物分子马达ATP合酶旋转催化合成。本项目拟紧密围绕生物分子马达ATP合酶的可控定向组装研究开展工作，通过模拟自然界叶绿体或线粒体的结构和功能，利用分子组装技术，构筑高效细胞能量工厂仿生体系。重点通过多级结构功能材料的可控制备与表/界面化学修饰，精准控制生物分子马达ATP合酶的组装方向，详细阐明表/界面微环境对ATP合酶分子排列的影响规律，建立这种生物大分子定向组装调控的新策略，探索组装体结构与性能的内在关系，加深生命能量转换过程的理解，结合人工质子梯度势的调节，以期实现生命能量转换的化学调控。

ATP is an energy substance used by cells for various life activities. It is mainly produced by the rotating biomolecular motor ATP synthase driven by the transmembrane proton gradient potential. This project intends to focus on the controllable assembly of biomolecular motor ATP synthase. By simulating the structure and function of chloroplasts or mitochondria in nature, highly-efficient bionic systems mimicking cell energy plant will be constructed via molecular assembly. Through the controllable preparation and surface chemical modification of hierarchical structured functional materials, the assembly direction of biomolecular motor ATP synthase can be precisely tuned. The effect of the surface microenvironment on the arrangement of ATP synthase will be elaborated in detail to develop new approaches for regulating directional assembly of this biomacromolecule. The inherent relationship between structure and performance of such assembled architectures will be explored to deepen the understanding of the life energy conversion process. Under the assistance of the adjustment of artificial proton gradient potential, chemical modulation of life energy conversion will be achieved.