污水处理发酵型聚磷菌Tetrasphaera的代谢途径及其与Ca.Accumulibacter的协同机制强化生物除磷

Ca.Accumulibacter一直被视为污水生物除磷的优势聚磷菌，近年来污水厂中发现以发酵型聚磷菌Tetrasphaera作为优势菌的除磷现象。该现象的生物学机制及优化调控是目前尚未解决的一大难题。.本研究以揭示发酵型聚磷菌Tetrasphaera的代谢途径及其与Accumulibacter的协同机制为目标。特色和创新在于①采用代谢组学和Raman-SIP-FISH技术原位识别并量化细胞水平的代谢产物，解析Tetrasphaera代谢途径与环境因子的动态响应机制；②在转录水平分析Tetrasphaera与Accumulibacter的协同机制和关键功能基因表达，揭示协同作用微生物学机理；③建立以Tetrasphaera作为优势聚磷菌的侧流EBPR工艺，通过Tetrasphaera与Accumulibacter的协同作用解决碳源不足和污泥排放量大的技术瓶颈，为污水强化生物除磷开辟新途。

Excessive discharge of wastewater in rich of phosphorus is the main cause of eutrophication of surface water. Strict discharge standards for phosphorus concentration into surface water have been set all over the word. On the other hand, the scarcity of phosphorus resources is a serious challenge in the world. Enhanced biological phosphorus removal (EBPR) is most commonly applied in wastewater treatment due to its sustainable and cost-effective characteristic, and also offers an opportunity for P-recovery. Candidatus Accumulibacter has been regarded as the dominant poly-phosphate accumulating bacteria (PAOs) in EBPR. Recently, the phenomenon of phosphorus removal, in which Tetrasphaera as the dominant bacteria, was found in the wastewater treatment plant. The biochemical mechanism and control of this phenomenon has not been clearly explained, which is one difficult problem to solve in the field of EBPR from wastewater...This study aims to reveal the metabolic pathway of Tetrasphaera, and the cooperative mechanism of Tetrasphaera and Candidatus Accumulibacter. The identity of Tetrasphaera cells and quantification of intracellular polymers under in situ conditions is carried out by metabonomics and Raman microspectroscopy-Stable isotope probing- Fluorescence in-situ hybridization (Raman-SIP-FISH) methods, in order to analyze the dynamic response of metabolic pathway of Tetrasphaera to environmental factors. Meanwhile, the cooperative mechanism of Tetrasphaera and Candidatus Accumulibacter, and expression of key functional genes is analyzed using reverse transcriptional quantification PCR (TR-QPCR) and cDNA high throughput sequencing at transcriptional level. Side-stream EBPR process with Tetrasphaera as dominant PAOs is developed to slove the problems of deficient carbon sources in wastewater and excessive waste activated sludge through the cooperative action of Tetrasphaera and Candidatus Accumulibacter, which provided a new way for EBPR from wastewater.