丙氨酸氨基转移酶调控褐飞虱适应抗虫水稻品种的代谢机制

抗虫作物品种的培育和应用是控制害虫危害的有效措施之一。但是，随着抗虫作物品种使用时间的延长，害虫能逐渐适应抗虫品种从而产生新的致害型，并导致抗虫品种的使用寿命缩短。迄今，害虫适应抗虫作物品种的代谢机制还很不清楚。申请者的前期研究表明，丙氨酸和丙氨酸氨基转移酶是褐飞虱适应抗虫水稻品种IR36的候选因子。本项目拟在此基础上，进一步明确丙氨酸氨基转移酶在褐飞虱适应IR36中的重要作用，并拓展至4-6个其他抗褐飞虱水稻品种；分析丙氨酸在褐飞虱体内的代谢途径，并研究褐飞虱抗性适应种群转化利用丙氨酸的代谢机制。创新之处在于：率先揭示褐飞虱利用丙氨酸氨基转移酶适应抗虫水稻品种的代谢机制。研究结果不仅有助于阐明害虫适应寄主植物的代谢机制，而且对延长抗虫水稻品种的使用寿命以及对褐飞虱的持续有效防控均具有重要意义。

As an effective control measure, breeding and utilization of resistant crop varieties plays important roles in the integrated management of insect pests. However, insect pests will gradually adapt to the resistant crop varieties with their continuously planting in the field. As a result, new virulence populations of the pests will emerge and lifetime of the resistant crop varieties will be shortened. Up to now, metabolic mechanisms of insect pests in the adaptation to resistant crop varieties are largely unclear. Our previous results showed that alanine and alanine aminotransferase (ALT) are involved in the adaptation of the brown planthopper (Nilaparvata lugens) to IR36, a resistant rice variety. Here, we will further clarify the role of ALT in the adaptation of the brown planthopper to IR36, as well as 4-6 other resistant rice varieties. Then, we will analyze metabolic pathways of alanine in the brown planthopper, and study metabolic mechanism of the adapted pest populations by utilizing alanine. The novelty of this proposal is to reveal for the first time the metabolic mechanism of the brown planthopper in the adaptation to resistant rice varieties through ALT. Our findings will not only contribute to clarify the metabolic mechanisms of insect pests adapted to resistant crop varieties, but also be of significance for prolonged service time of resistant rice varieties and sustainable management of the brown planthopper.