U1剪接体特异性蛋白U1C参与植物开花的转录后调控机制研究

开花决定植物分布和地理适应性，对提高农作物产量和品质、增强作物抗逆性和扩大作物适应性具有重要生物学意义。近年来，关于植物开花的转录后水平调控研究逐渐成为热点问题之一，尤其是选择性剪接调控。深入研究选择性剪接调控植物开花的分子网络，将有助于解答选择性剪接如何调节植物适应性发育以响应外界环境等科学问题。本团队前期研究工作发现U1剪接体亚基u1c突变体在长日照条件下呈现生长发育缺陷和早花表型，但U1C在开花调控中的具体功能和下游靶基因仍不明确，在此基础上，本项目拟通过研究：（1）U1C在植物开花调控中的具体功能；（2）U1C与SC35的互作机制及遗传关系；（3）U1C与SC35互作介导SUPPRESSOR OF OVEREXPRESSION OF CO1（SOC1）的选择性剪接调控植物开花的分子机制，以期完善植物开花的转录后调控网络，扩展人们对植物繁衍和环境适应性发育理论的科学认知。

Flowering determines the plant distribution and geographical adaptability, which has pivotal roles for improving crop yield and quality, stress resistance and crop adaptability. Until now, most works are focused on the transcriptional regulation of flowering, however, post-transcriptional regulation is less explored, especially in the alternative splicing. Our preliminary experiment showed that mutation of spliceosomal protein U1C resulted in the growth defects and early flowering in Arabidopsis under long day conditions. However, the function of U1C and its downstream target genes are remained unknown in the flowering pathway. According to these data, we aimed to identify the flowering gene network regulated by U1C, and illustrate the interaction mechanism and genetic relationship between U1C and SC35. Meantime, alternative splicing of SUPPRESSOR OF OVEREXPRESSION OF CO1 (SOC1) mediated by the interaction between U1C and SC35 was further explored. Collectively, our study provides insight roles in the post-transcriptional regulation network of flowering, and expands the understanding of plant reproduction and environmental adaptive development.