马尾松磷转运蛋白基因PmPT1的低磷胁迫应答及互作蛋白解析

植物磷利用率取决体内与磷吸收、转运等生理活动相关蛋白的协同调控，磷转运蛋白在低磷胁迫应答中具有重要地位，有研究表明它可能需要和其他蛋白互作才能发挥正常的生理功能，但这一现象的研究缺乏理论基础。根据我们前期研究工作，以磷转运蛋白PmPT1为切入点，检测不同马尾松种质中PmPT1表达及生长、生理生化变化，探明PmPT1与耐低磷的相关性。构建植物表达载体，研究PmPT1时空表达谱和应对胁迫的差异表达调控，结合生理生化指标，利用免疫细胞化学法确定其组织分布，鉴定PmPT1耐低磷功能。同时利用GST-pulldown和免疫共沉淀法筛选PmPT1的互作蛋白，鉴定互作蛋白在PmPT1表达和磷积累中的功能，阐明PmPT1对低磷胁迫的应答。本研究旨在鉴定PmPT1耐低磷功能，发掘其互作蛋白，丰富植物低磷胁迫应答的分子和生理基础，同时为马尾松育种改良利用提供基因资源和理论依据。

The utilization of plant phosphorus depends on the synergetic regulation of proteins linking phosphorus element absorption, transport and physiological activity. Thererfore, phosphorus transporters play an important role in all activities. And normal physiological functions of phosphorus transporters need to interact with other protein. In our previous showed that gene expression, growth and variation physiological and biochemical of different germplasm Pine Masson were detected by used PmPT1 as to tangent point, in order to understand the relationship between PmPT1 and low phosphorus tolerance. Function of PmPT1 toleranced low phosphorus was identied by constructed plant expression vector, studying spatiotemporal PmPT1 expression spectrum and genetic differential expression combined with seedling growth and physiological and biochemical indexes, determining the distribution of PmPT1 protrin in tissue by immunocytochemistry. The interaction proteins of PmPT1 were screened by GST-pulldown and immunocoprecipitation, and the protein functions of PmPT1 expression and phosphorus accumulation were indentied understand deficient-Pi. In this study, PmPT1 function of toleranced low phosphorus will be identied and the interaction protein be revealed in order to enrich the molecular and physiological basis of adaptability to phosphorus deficiency Stress, improvement of masson pine breeding and provide a reliable theoretical basis for using.