水稻ALOG基因家族功能的研究: G1L2与G1L6及G1冗余性地参与调控水稻内外稃及不育外稃的发育的机理

水稻ALOG基因家族共有十个成员（G1及G1L1~G1L9），其中G1、G1L5 和G1L6的功能已被研究，它们都参与了水稻花发育的过程，而其它7个成员功能尚属未知。本项目通过构建所有10个成员的基因敲除株系、多个双突变，三突变株系，多个过表达株系及多个嵌合抑制基因沉默株系来研究ALOG基因家族成员的功能和冗余性。值得注意的是，通过嵌合抑制子基因沉默的技术抑制G1L2及其冗余基因的下游靶标，转基因植株表现出与g1l6突变体及g1突变体相似的表型，即内外稃变窄，部分小花不育外稃异常发育。说明G1L2与G1L6及G1冗余性地参与调控水稻内外稃和不育外稃的发育。因此，我们将进一步研究深入研究G1L2、G1L6和G1的表达模式和基因功能，揭示这三个基因冗余性地调控水稻内外稃及不育外稃发育的机理。通过该项目的研究，将有助于我们进一步了解水稻花发育的分子生子生物学机制。

Members of the Arabidopsis LSH1 and Oryza G1 (ALOG) family of protein had been shown to function as key developmental regulator in land plants. In rice, there are 10 members of ALOG family (G1, G1L1~G1L9). Although 3 of them (G1, G1L5 and G1L6) had been characterized as transcription factors involved in rice flora development, the biological function of the rest family members still remain unknown...In this project, we mainly focus on the uncharacterized ALOG members. To understand the biological function of these members, we generated gene knock out line for each member sing CRISPER/Cas9, we also generated the gene overexpression line for all these members. ..We did not observe any developmental defective phenotype for knocking out the uncharacterized ALOG members. Considering the sequence similarity among the members, double mutant lines and chimeric repressor gene-silencing lines were generated in order to study the redundancy of these members...It was notable that by repressing the targets of G1L2 and its functionally redundant transcription factors using chimeric repressor gene-silencing technology, the transgenic plant showed an abnormal development of floret with slender lemma/palea, sometimes with elongated sterile lemmas, indicating that G1L2, G1L6 and G1 may involve in the regulation of rice lemma/palea and sterile lemmas redundantly. Therefore, we mainly focused on the genetic interaction among these genes...This research plan will make us known more about the rice ALOG gene family, especially on how G1L2, G1L6 and G1 redundantly regulate the development of lemma/palea and sterile lemmas. Our study may shed some light on the molecular mechanism of the rice flora development, which is critical for the yield.