雌二醇在三疣梭子蟹卵巢甘油三酯(TAG)积累过程中的功能研究

雌二醇是甲壳动物体内活性最高的雌激素，参与其卵巢发育和脂质代谢过程。本项目在探明三疣梭子蟹卵巢发育过程中雌二醇和甘油三酯(TAG)含量变化、外源雌二醇影响其肝胰腺脂质代谢的基础上，拟系统研究“雌二醇在三疣梭子蟹卵巢TAG积累过程中的功能”。项目首先通过共定位研究卵巢中TAG和雌二醇的相关关系；在此基础上研究外源雌二醇等激素在离体和活体条件下对雌体肝胰腺和卵巢中TAG代谢相关基因的表达、酶活力及脂质组成的影响，并通过转录组和脂质组筛选雌二醇调控TAG代谢的差异基因和脂质分子；基于以上结果，构建雌二醇调控TAG代谢的可能路径，筛选1-2个重要调控靶基因(SREBP/LpR)进行功能验证，进而全面评估雌二醇对三疣梭子蟹外源性卵黄合成期TAG代谢的作用及可能途径。预期研究结果不仅可深入了解雌二醇调控三疣梭子蟹TAG代谢的分子机制，同时也可为研究甲壳动物生殖调控和内分泌干扰物的毒理机制提供基础资料。

Estradiol is the most active estrogen in crustaceans, and it participates in ovarian development and lipid metabolism. Our previous studies have explored the changes of estradiol and TAG content during the ovarian development of the swimming crab Portunus trituberculatus, and the exogenous estradiol can affect its lipid metabolism in hepatopancreas. The current project is designed to comprehensively investigate the role of estradiol in regulating the triglyceride (TAG) accumulation in the ovary of P. trituberculatus. Firstly, the co-localization analysis of TAG and estradiol in the ovaries will be performed to reveal their correlation. Then the in vitro and in vivo experiments will be used to study the effect of exogenous estradiol and other hormones on the expression level of TAG metabolism-related genes, enzyme activity and lipid composition in female hepatopancreas and ovaries, and to analyze the key TAG metabolism genes and lipid molecules regulated by estradiol through transcriptome and lipidome. Based on the above results, the regulation pathway of estradiol in TAG metabolism during the ovarian development of P. trituberculatus will be preliminarily constructed; 1-2 important regulatory target genes (SREBP/LpR) will be screened for functional verification; thereby comprehensively evaluate the effect and possible pathway of estradiol on TAG metabolism during the exogenous vitellogenic stage of P. trituberculatus. The research results not only provide in-depth understanding of the molecular mechanism of estradiol regulating TAG metabolism in P. trituberculatus, but also provide basic information for the study of crustacean reproduction regulation and the toxicological mechanism of endocrine disruptors.