Coiled-coil结构域编码基因DMS1参与水稻减数分裂和花粉发育的分子机理

水稻花粉发育是一个复杂的生物学过程，其发育缺陷往往会导致雄性不育。完全的雄性核不育基因在理论和应用上都有较大的研究价值。我们获得了一个水稻突变体dms1，经多年观察鉴定，确定其小孢子母细胞减数分裂过程出现异常、花粉发育缺陷，表现出完全的雄性不育。图位克隆及互补验证确定了DMS1目的基因，其编码一个coiled-coil结构域。DMS1是一个未报道过的新基因，其蛋白定位在细胞核、且具有转录激活活性。本研究将确定dms1小孢子母细胞减数分裂异常和花粉缺陷的理化及免疫原因；明确DMS1的表达模式；筛选及验证DMS1的互作蛋白及下游调控基因，并最终揭示DMS1的基因功能及其参与水稻减数分裂和花粉发育的分子机制。

The development of pollen is a complicated cytological process in rice, and any abnormal behaviors in this process would result in male sterile. Therefore, the molecular mechanism analysis of male gamete development is a research hotspot at present. A rice mutant dms1 was identified from EMS-treated Jinhui10 showed no apparent difference between the wild type in vegetative stage. But in reproductive stage, the meiosis of pollen mother cells and pollen were abnormal in dms1, and finally caused complete male sterility. dms1 phenotype was controlled by one recessive nuclear gene. By mapping and complementary verification, one gene encoding a coiled-coil domain protein was selected as the candidate gene. DMS1 is a novel gene in rice. In addition, DMS1 located in the nucleus and had transcriptional activation activity. Based on above results, this research aims to investigate the physical, chemical and immune causes of abnormal meiosis behavior and pollen in dms1, to identify the expression pattern of DMS1, to screen out proteins interact with DMS1 and downstream regulatory genes by DMS1. Those exploration will provide a foundation to study the molecular mechanism of meiosis and pollen development in rice.