番茄黄化曲叶病毒协同烟粉虱传播番茄褪绿病毒的效应与机制

媒介昆虫在许多植物病害的蔓延过程中起到关键作用。近年来番茄褪绿病毒（ToCV）在中国内陆的暴发成灾与其传播媒介烟粉虱具有直接联系。田间调查发现发病地区番茄存在ToCV与番茄黄化曲叶病毒（TYLCV）复合侵染现象，并且前期研究发现Q烟粉虱携带TYLCV明显促进了其对ToCV的获取。本项目围绕“TYLCV协同烟粉虱传播ToCV的效应与机制”这一科学问题，明确TYLCV对Q烟粉虱传播ToCV能力的影响，进一步通过刺吸电位技术研究其取食行为机制；解析TYLCV和ToCV复合侵染对Q烟粉虱寄主适应性的影响；利用色谱、酶活性测定、实时荧光定量等技术揭示植物和Q烟粉虱应对TYLCV和ToCV复合侵染的内在生理响应机理。研究结果将增强对于烟粉虱传播新入侵病毒ToCV特性的认识，深化对植物病毒-植物-昆虫三者互作机理的探索，为新形势下开展多病毒流行的科学防控和媒介昆虫的有效治理提供理论依据。

Insect vectors can always play a key role in the expansion of many plant diseases. In recent years, epidemics of Tomato chlorosis virus in many regions of China inland could be attributed to the outbreaks of its vector Bemisia tabaci. The cases of mixed infection of ToCV and Tomato yellow leaf curl virus (TYLCV) in tomato plants was detected by field investigation. The previous studies also found that bringing TYLCV could obviously improve the acquisition ability of B. tabaci for ToCV. However, the influence on the B. tabaci transmission of ToCV brought by TYLCV and the mechanism still remain unknown. This study focus on the above questions, using behavior, physiology, biochemistry and molecular biology technologies to conduct the following researches: 1) Assess the effect on the transmission ability of B. tabaci Q brought by TYLCV, and further clarify the feeding behavior; 2) Examine the host adaptability change of B. tabaci Q caused by the mixed infection of TYLCV and ToCV; 3) Reveal the physiological response of both plant and B. tabaci Q to the mixed infection of two virus through chromatography, determination of enzyme activity and real-time quantity PCR. The results will increase our knowledge about B. tabaci transmission characteristics of ToCV, facilitate our exploration of the interaction mechanism of plant virus-plant-insect. Furthermore, this study also will provide basic information for the scientific prevention of multiple plant virus and effective management of vector insects.