西花蓟马对不同食物营养效应的响应机制

西花蓟马是危害花卉的重要害虫，具有种群增长快和繁殖能力强的特点。前期研究结果表明该虫取食花粉花蜜、及螨类卵的杂食性有利于种群的增长和繁殖，但作用机制尚不清楚。本项目以杂食性促进西花蓟马种群暴发为科学问题，以取食玫瑰叶片（植食性）、无花粉花蜜的玫瑰花瓣（植食性）、包含花瓣和花粉花蜜的玫瑰花（植食性+花粉花蜜）、二斑叶螨为害的玫瑰叶片（植食性+肉食性）4种食物的西花蓟马为研究对象，通过生态学的方法研究蓟马取食不同食物后体长体重、卵巢发育、生长发育、繁殖力和生命表参数的异同；通过生化分析揭示其取食不同食物后体内营养物质、消化酶种类及活性、卵黄原蛋白含量的变化、及与不同食物营养物质的相关性；采用转录组明确体内消化酶基因及卵黄原蛋白基因表达水平的差异；通过RNAi技术验证这些差异基因在适应不同食物中的作用。以期阐明杂食性促进西花蓟马生长发育与生殖的营养效应，为有效防控西花蓟马种群暴发提供理论依据。

Frankliniella occidentalis is an important pest on flowers, which has the characteristics of rapid population growth and strong reproductive capacity. Previous studies have shown that the omnivorous characteristics of feeding pollen nectar and mite eggs are very important for triggering its growth and reproduction, but the mechanism is not clear. In this project, rose leaves (phytophagous), rose petal (phytophagous), rose flower (phytophagous+feeding pollen nectar), rose leaves damaged by Tetranychus urticae (phytophagous + carnivorous) will be chosen as 4 kinds of food. The differences of individual size, pupa weight, growth and development, fecundity and life table parameters of F. occidentalis will be compared by ecological methods. The changes of nutrients, the activities of digestive enzyme, ovarian development, vitellogenin content will be compared. The correlation between index of thrips with different kinds of food nutrients will be revealed. Transcriptome will be used to study the expression levels of digestive enzyme gene and vitellogenin gene. RNAi technology will be used to verify the role of digestive enzyme gene and vitellogenin gene in adaptation to different kinds of food. The purpose of this study is to elucidate the nutritional effects of food on the growth, development and reproduction of omnivorous F. occidentalis. The results will provide theoretical basis for controlling the outbreak of F. occidentalis population.