参与核糖体rRNA修饰的非编码RNA uhg5基因对果蝇睡眠调控机制的研究

睡眠是许多动物包括昆虫普遍存在的生理活动，睡眠紊乱对昆虫生命活动有严重影响。睡眠是神经活动调控的过程，蛋白质的动态调控在神经活动中非常重要。核糖体作为蛋白质合成的机器，其合成动态在睡眠调控过程中有何作用是一个重要的生物学问题。本项目围绕这个问题展开研究，我们初步研究发现，参与核糖体rRNA修饰的非编码RNA uhg5基因在睡眠调控中有关键作用。本项目拟进一步从其作用于睡眠的具体过程和下游作用分子机制两方面，探究uhg5基因对睡眠的作用机理；同时，从其上游表观遗传的调控机制和昼夜差异表达的调控机制两方面，探索uhg5基因的上游调控机制。本项目的开展将有助于阐明睡眠过程中的核糖体生物合成的调控机制和作用，并深入探讨通过表观遗传调控机制整合的上游信号如何作用于核糖体功能调控。该项目的实施将为核糖体水平的基因表达调控机理带来全新的理解。

Sleep is a common physiological activity in many animals, including insects. Sleep disorder has a serious impact on insect life activities. Sleep is a process of neural activity regulation, and the dynamic regulation of protein is very important in neural activity. Ribosome, as a protein synthesis machine, the role of ribosome synthesis dynamics in sleep regulation is an important biological problem. This project focuses on this problem. Our recent preliminary study found that the non-coding RNA uhg5 gene involved in ribosomal rRNA modification plays a key role in sleep regulation. This project intends to further explore the mechanism of the function of uhg5 gene on sleep from its specific aspects of function on sleep and its downstream molecular mechanism; at the same time, it explores the upstream regulatory mechanism of uhg5 gene from its upstream epigenetic regulatory mechanism and the regulatory mechanism of day night differential expression. The development of this project will help to clarify the regulatory mechanism and role of ribosome biosynthesis in the process of sleep, and further explore how upstream signals integrated through epigenetic regulatory mechanism affect the regulation of ribosome function. The implementation and completion of the project will bring a new understanding of the regulatory mechanism of gene expression at ribosome level.