荧光/电化学双信号模式生物传感检测平台的构筑及其在食品中抗生素多残留分析中的应用

复杂食品样品基质中抗生素的可靠分析具有挑战性，发展简便快捷、灵敏可靠的抗生素多残留传感检测新技术具有重要的研究意义和实际应用价值。双信号模式传感体系可提高实际应用时传感检测的灵敏度和准确性，而基于不同类型信号体系的双模式传感器设计存在瓶颈。本项目以重要的目标抗生素为靶标，重点围绕食品样品前处理、荧光/电化学双模式信号标签的构建、双模式传感检测平台的设计展开研究，利用适配体分子识别作用，配合磁性分离技术构筑荧光/电化学双模式生物传感检测平台，从分子识别、信号发生和信号分辨等多角度、多层面阐明靶向传感机理。本项目聚焦食品安全快速检测中实际问题，致力于突破荧光/电化学双模式传感器的设计瓶颈，以实现抗生素的荧光/电化学双模式多残留传感分析，拓展双模式传感器在食品安全检测领域的应用范围。本项目的开展对我国食品安全现场快速检测技术的发展具有积极的推动作用，可以为抗生素超标产品的监管和溯源提供技术支持。

The reliable analysis of antibiotics in food samples with complex matrix is challenging, and the development of simple, fast, sensitive, and reliable sensing technology for the detection of antibiotic multiresidue has important research significance and practical application value. The dual-mode signal sensing system can improve the sensitivity and accuracy of sensing detection in practical applications, but the dual-mode sensor design based on different type of signal systems has bottlenecks. This project takes important target antibiotics as the target, and focuses on the construction of fluorescent/electrochemical dual-mode signal tags, the design of dual-mode sensing platform and the research of food sample pretreatment. A fluorescence/electrochemical dual-mode biosensing platform based on aptamer molecular recognition and magnetic separation would be constructed. The project will clarify the mechanism of targeted sensing from multiple angles and levels, such as molecular recognition, signal generation, and signal resolution. This project is focusing on the practical problems in rapid food safety testing, and is committed to breaking the bottleneck of the design of fluorescence/electrochemical dual-mode sensor to achieve the fluorescence/electrochemical dual-mode multiresidue sensing analysis of antibiotics and expand the application of dual-mode sensor in food safety testing. The development of this project has a positive role in promoting the development of on-site rapid detection technologies for food safety in China, and can provide technical support for the supervision and traceability of products with excessive antibiotics.