自噬在系统性坏死植物病毒互作体系中的作用研究

系统性坏死（SN）是植物的一种毁灭性病害，细胞和组织的系统性坏死既不利于植物的生存也不利于活体寄生的病毒存活，但其发生、形成及调控的机制尚不明确。我们前期首次从番茄上分离到一个辣椒脉斑驳病毒（ChiVMV），可侵染烟草、番茄、辣椒等茄科植物导致寄主的系统性坏死。进一步研究发现该病毒的RNA沉默抑制子与烟草的过氧化氢酶互作，影响宿主的活性氧代谢、促进病毒的侵染。受侵染植物虽然发生了类似超敏反应和程序性细胞死亡的响应，但SN的病程发展结果使植物、病毒两败俱伤。本项目拟在此基础上，探究植物系统性坏死的调控机理。利用ChiVMV侵染性克隆，结合Y2H、BiFC、GST pull-down、VIGS、CRISPR/Cas9等技术，从病毒因子和宿主基因两个方面探究自噬在SN症状形成中的作用。本项目的研究结果将有助于深入理解自噬在病毒与寄主博弈中的功能，也为病毒引起的植物系统性坏死研究提供新的科学证据。

Systemic necrosis (SN) is a devastating disease of plants. Systemic necrosis of cells and tissues is not conducive to the survival of plants or the survival of living parasitic viruses, but the mechanism of its occurrence, formation and regulation remains elusive. We previously isolated a Chilli vein mottle virus (ChiVMV) from tomato for the first time. It could infect tobacco, tomato, pepper and other Solanaceae plants and caused systemic necrosis of the hosts. Further study showed that the RNA silencing suppressor of the virus interacted with catalase of tobacco, which affected the active oxygen metabolism of the host and facilitated the infection of the virus. Although the infected plants exhibited similar responses of hypersensitive response and programmed cell death, the development of SN resulted in both plant and virus failure. On this basis, the project intends to explore the regulatory mechanism of plant systemic necrosis. The role of autophagy in the formation of SN symptoms will be analyzed from two aspects of virus factor and host gene by using ChiVMV infectious clone, Y2H, BiFC, GST pull-down, VIGS, CRISPR/Cas9 and other technologies. The results of this project will help us to understand the function of autophagy in the arm-race between virus and host, and will provide new scientific evidence for the study of systemic necrosis of plants caused by virus.