从LncRNAs调控TLRs/NF-κB信号通路角度探讨热补针法治疗RA的抗炎机制

类风湿关节炎（RA）是一种严重危害人类健康的常见疾病，目前尚无特效治疗药物，针灸治疗优势明显。现已证实持续性炎症反应是RA发展和加重的最主要病理机制，激活TLRs/NF-kB信号通路是RA炎症反应发生和持续的关键，而 LncRNAs调控TLRs/NF-kB信号通路是抑制RA炎症反应的内源性关键机制。前期研究显示热补针法治疗RA疗效肯定，具有显著的抗炎作用，但是分子机制不明。基于此，建立“热补针法的抗炎机制与其通过调控LncRNAs的表达而抑制TLRs/NF-κB信号通路活性密切相关“的假说，采用卵蛋白诱导联合低温冷冻法建立RA家兔模型，运用基因芯片、qRT-PCR、Western Blot等技术系统研究热补针法治疗RA的抗炎机制，从LncRNAs调控TLRs/NF-κB信号通路角度阐明热补针法抗炎的分子机制，为热补针法治疗RA提供可靠的理论依据。

Rheumatoid arthritis (RA) is a common disease that seriously endangers human health. There is no specific treatment before, but acupuncture has obvious advantages. It has been confirmed that persistent inflammatory response is the most important pathological mechanism for the development and aggravation of RA, activation of TLRs/NF-kB signal pathway is the key to the occurrence and persistence of RA inflammatory response, and LncRNAs regulation of TLRs/NF-kB signal pathway is the endogenous key mechanism to inhibit RA inflammatory response. Previous studies have shown that heat-reinforcing acupuncture is effective in the treatment of RA and has significant anti-inflammatory effect, but the molecular mechanism is unknown. Based on this, we established the hypothesis that the anti-inflammatory mechanism of heat-reinforcing acupuncture is closely related to the inhibition of TLRs/NF- κ B signal pathway activity by regulating the expression of LncRNAs. The rabbit model of RA was established by ovalbumin induction and cryopreservation. The anti-inflammatory mechanism of heat-reinforcing acupuncture treatment of RA was studied systematically by gene chip, qRT-PCR, WesternBlot and other techniques, and the molecular mechanism of heat-reinforcing acupuncture anti-inflammation was clarified from the point of view of LncRNAs regulating TLRs/NF- κ B signal pathway. The purpose of this study is to provide a reliable theoretical basis for the treatment of RA with heat-reinforcing acupuncture.