RLP23-SOBIR1复合体介导连作地黄免疫应答紊乱的分子机制

连作障碍是中药材生产和资源可持续发展的重大难题。植物免疫识别受体能感知外界胁迫并激活下游免疫响应。项目前期发现免疫应答紊乱是导致地黄连作障碍的重要原因，LRR-RLP23与其共受体激酶SOBIR1广泛参与了该过程。据此，本项目拟研究：①在测定连作地黄专化致病菌候选效应蛋白侵染效应的基础上，鉴定LRR-RLP23特异性识别的效应因子；②利用酵母双杂交、GST pull-down和BiFC技术确证RLP23-SOBIR1复合体及其下游靶标蛋白；③利用反向遗传学结合地黄受害表型特征，明确RLP23-SOBIR1与下游靶标蛋白的功能依赖性关系。研究最终将阐明RLP23-SOBIR1复合体介导连作地黄免疫应答紊乱的分子机制，为通过免疫锻炼的方式弥补和提升连作地黄抵御专化病原菌侵害的能力、深化中药资源生态学和分子生药学研究、实现中药材生产的可持续发展提供理论依据和技术支撑。

Continuous cropping obstacle is a major problem in the production and the sustainable development of Chinese medicinal herbs. Plant immune recognition receptors can sense external stimuli and activate downstream immune responses. Our previous study showed that the immune response disorder is an important reason for the continuous cropping obstacle of Rehmannia glutinosa Libosch, and that LRR-RLP23 and its co-receptor kinase SOBIR1 participate in this process. Based on this, the project will: (1) identify the effector specifically recognized by LRR-RLP23 on the basis of determining the infective effect of candidate effector proteins of specialized pathogens in the continuous cropping of R. glutinosa; (2) identify the RLP23-SOBIR1 complex and its downstream target proteins by BiFC technology; (3) clarify the functional dependence relationship between RLP23-SOBIR1 and downstream target protein using reverse genetics combined with R. glutinosa phenotypic characteristics. The project will eventually clarify the molecular mechanism of RLP23-SOBIR1 complex mediated immune response disorder in continuous cropping of R. glutinosa. It will also provide the theoretical basis and technical support for improving the resistance level of replanted R. glutinosa against specialized pathogens through immunological training, deepening the research of Chinese medicine resource ecology and molecular pharmacy, and achieveing sustainable development of Chinese medicine production.