基于炎症免疫网络调控的新安固本培元法之方证对应改善COPD患者感受的分子机制研究

慢性阻塞性肺疾病（COPD）是以气流受限不完全可逆为特征，慢性炎症与免疫机制相互交织、免疫与代谢互相作用的渐进性加重疾病。依新安医学固本培元理论指导治疗COPD疗效确切，可能通过巩固先天免疫屏障，培护后天免疫系统，从而调控炎症免疫代谢网络，延缓气道功能障碍发挥作用，但方证如何相应及患者感受改善机制待进一步明确。鉴此，我们以新安经典方药（温肺汤、补中培元汤、六味补气方）改善COPD稳定期肺气虚证、肺脾气虚证、肺肾气虚证患者感受为切入点，运用细胞因子抗体芯片、流式细胞微球芯片捕获技术、代谢组学等现代技术探寻改善患者感受的生物学实质，构建“固本培元方药—证候要素—免疫炎症网络—代谢轮廓—患者感受”多因子复杂网络模型，通过网络聚类与动力学计算方法深度挖掘新安医学固本培元法改善COPD患者感受的关键策略，以此桥连病证结合模型，进一步验证固本培元法方证对应改善患者感受的分子机制，为临床治疗提供新途径。

Chronic obstructive pulmonary disease (COPD) is a progressive disease characterized by incompletely reversible airflow limitation, in which chronic inflammation and immune mechanisms are intertwined and immunity and metabolism interact. The treatment of COPD is effective with the guidance of Xin'an medical's method of “Guben Peiyuan”, which may consolidate the innate immune barrier and protect the acquired immune system to regulate the inflammation-immunity-metabolism network to delay airway dysfunction of COPD. However, the way that formulas and syndromes in traditional Chinese medicine (TCM) correspond to each other, the molecular mechanism of the improvement of self-perception, and their associations remain to be further clarified. Therefore, we select Xin'an medical's classic formulas, including Wenfei Decoction, Buzhong Peiyuan Decoction, and Liuwei Buqi Recipe which have been proved could improve the self-perceptions of lung qi deficiency, lung and spleen qi deficiency, and lung and kidney qi deficiency stable patients of COPD, respectively, as a starting point, apply modern technologies including cytokine antibody array, cytometric beads array, metabolomics to construct a multi-factor complex network model of “ ‘Guben Peiyuan’ Formulas-Immunity and Inflammation Network-Metabolic Profile-Self-perception” to explore biological practices of the improvement of self-perception. Furthermore, we will deeply explore the key strategies of Xin'an medical's method of “Guben Peiyuan” to improve the feelings of COPD patients through network clustering and dynamic calculation methods, which bridge the COPD model of combine disease and symptom complex, and further verify the molecule mechanisms of consolidating basis and cultivating primordial qi method's classic formulas correspond in the improvement of self-perceptions, to provide new strategies for clinical treatment of COPD.