基于RAD-seq的云南黑籽南瓜居群遗传结构及起源扩散演化研究

黑籽南瓜是云南特色资源，产量极高，具有很强的抗寒、抗旱、抗病、抗贫瘠性，特别对瓜类枯萎病具高抗性，具有十分重要的生产价值及科研价值。然而黑籽南瓜地方品种较多，形态差异大，生产较为混乱，品质有优有劣，资源分类不明晰。如何揭示黑籽南瓜地方品种/居群的遗传多样性水平、阐明黑籽南瓜地方品种/居群之间的遗传关系，是有效利用这一资源的前提。本研究将对云南黑籽南瓜资源进行全面考察，对分布区的代表性地方品种/居群进行取样，利用限制位点相关的简化基因组测序方法(RAD-seq)，获取大量的SNP标记，通过群体遗传学与进化生物学相关分析手段评价云南黑籽南瓜的遗传多样性、居群遗传结构、地方品种/居群间的亲缘关系。通过与祖先类群对比，构建高分辩率的进化树，深入探讨黑籽南瓜的起源扩散演化路径，厘清古老品种与进化类群。本项目的实施有助于科学有效地发展黑籽南瓜这一特色资源，具有重大实践价值和理论意义。

Cucurbita ficifolia Bauche is a characteristic resource of Yunnan province, C. ficifolia has high yield and strong cold resistance, drought resistance, disease resistance, barren resistance, especially has high resistance to cucurbits fusarium wilt, so it has very important production and scientific research value. However, there are lots of local varieties and big morphological differences, so the production of C. ficifolia is chaotic, the quality is instability, and the classification of resources is not clear. How to reveal the genetic diversity level and elucidate the genetic relationship between the landraces/populations of C. ficifolia is the premise of effective utilization of this resource. In this study, we will apply field investigation, and collected represented resources of C. ficifolia. Applying restriction-site associated DNA sequencing (RAD-seq), we can acquire a large number of SNP tags, combine analyses methods of population genetics and evolutionary biology, investigate genetic diversity, population genetics, relationship of landrace/populations were explored. Through the comparison with the ancestral groups, construct a high resolution evolutionary tree to dissect the origin location and evolutionary path of C. ficifolia. Clarify ancient landrace and evolutionary population. The implementation of this project is conducive to the scientific and effective development of C. ficifolia, which has great practical value and theoretical significance.